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Title : DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR ESTIMATION OF MILNACIPRAN HCL IN PHARMACEUTICAL FORMULATIONS
Authors : *Mubarakunnisa Md, Prameela Rani A and Seelam Harika
Abstract : A new simple isocratic RP-HPLC method was developed for the determination of milnacipran in bulk drug and in its capsules formulations. The mobile phase selected was methanol: acetonitrile:0.1% O-phosphoric acid in the ratio of 55:35:10%(v/v) and Zodiac C18 column with dimensions (250X4.6mm, 5µ)was used as stationary phase column temperature is ambient throughout the method. The flow rate and wave length observed were 1.0 mL/min and 220nm, respectively. The method was validated and it was concluded that the developed method was accurate, sensitive, precise, robust, and useful for the quality control of milnacipran in bulk drug and pharmaceutical preparations (capsules).

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