||Traditional medicines have long been used by the Moroccan people. Several medicinal recipes prepared from a mixture of plants are often used by traditional medicinal practitioners for the treatment of many diseases including cancer. However, little is known for their scientific information especially their anticancer effects. In this study, one traditional used Moroccan herbal remedy for treatment of hemorrhoids and cancer therapy (cervical cancer, skin cancer) was investigated for its cytotoxic effect against cancer cell lines. Aqueous herbal distillate of mixture in equal quantity of five medicinal plants was evaluated for its in vitro cytotoxicity, using the 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, against two human cancer cell lines; cervix adenocarcinoma (HeLa) and breast cancer cell line (MCF7). The mixture of plants exhibited potent cytotoxicity with an IC50 of 60 µg/ml for Hela cells and 122 µg/ml for MCF7 cells. Furthermore, the mixture was evaluated in vitro for antioxidant potential with the spectrophotometric method based on the reduction of the stable DPPH (1,1-diphenyl-2-picryl hydrazyl) free radical. The IC50 value was calculated in order to evaluate the antioxidant efficiency of plants mixture. The Aqueous herbal distillate of mixture exhibited moderate antioxidant activity 62.76 %, with an IC50 value of 96.8 µg/ml compared to the IC50 value of 4.73 µg/ml as shown by the reference antioxidant Trolox. In conclusion, this herbal remedy that was used to treat cancer patients has effective cytotoxic effect on cancer cells in vitro, and further studies of the active extracts are necessary for chemical characterization of the active compounds and more extensive biological evaluations.