||A simple, accurate and precise UV Spectroscopic method was developed for the simultaneous estimation of Duloxetine Hydrochloride and Methylcoblamin. The overlay spectra of Duloxetine hydrochloride and Methylcoblamin exhibit λ max of 291 nm and 350 nm for Duloxetine hydrochloride and Methylcoblamin in Double distilled water respectively. The drugs obeyed the Beer’s law in the range of 8-28μg/ml and 0.6-15μg/ml for Duloxetine hydrochloride and Methylcoblamin with correlation coefficients of 0.998 and 0.999 respectively and it has showed good linearity. The results of analysis were validated by recovery studies. The % recovery was found to be 99.99-100.96 % for Duloxetine hydrochloride and 100.55-101.02 % for Methylcoblamin. LOD and LOQ were found to be 1.278μg/ml, 2.330μg/ml for Duloxetine hydrochloride and 0.949μg/ml, 2.857μg/ml for Methylcoblamin respectively. The %RSD values were less than 2. The method was found to be simple, accurate, precise, economical and reproducible.