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Title : RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF CEFEPIME AND TAZOBACTAM IN MARKETED FORMULATION
Authors : *M. Bhavana, T. RamamohanaReddy, M. Sandhya, V. Uma Maheswara Rao
Abstract : A new precise, accurate, reliable validated method for the determination of Cefepime and Tazobactam has been developed by using reverse phase high performance liquid chromatography (RP-HPLC) in pharmaceutical dosage form. Chromatographic separation was carried out by using mobile phase 0.02M Potassium dihydrogen phosphate: Acetonitrile (95:5v/v, pH-3.0 adjusted with Orthophosphoric acid) on Sunfire C18 (50 x 4.6 mm, 5 µ) at a flow rate 0.8ml/min with UV detection at 220nm.The retention times for Cefepime and Tazobactam were 2.243 and 4.910 min respectively and both drugs showed good linearity in the range of 250-750 µg/ml and 31.25-93.75 µg/ml. The proposed method has been successfully applied to pharmaceutical formulation and was validated according to ICH guidelines and method showed good precision with percentage relative standard deviation less than 2%. The percentage recovery for Cefepime and Tazobactam was found between 99.80-101.11% and 100.43-101.14% respectively indicating the proposed method was accurate and precise.

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