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Title : ISOLATION, QUANTIFICATION AND ANTIMICROBIAL ACTIVITIES OF PHYTOSTEROLS FROM DIFFERENT PARTS OF CASSIA PUMILA LAMK
Authors : Ankita Yadav, Richa Bhardwaj and R. A. Sharma
Abstract : Cassia species have been of keen interest in phytochemical and pharmacological research due to their excellent medicinal values. Aim of this study is to identify and characterize the bioactive principles from the different parts of Cassia pumila. Four compounds were isolated and purified and the structures were determined as β-sitosterol, lanosterol, campersterol, stigmasterol by analysis of physical, chemical and spectral characterstics (IR,NMR). Present study shows the presence of various concentrations of phytosterols in different plant parts of C. pumila. The higher level of total phytosterols was measured in pods of C. pumila (1.15 mg/g dw) and lowest in flowers (0.38mg/gdw).The highest level of β-sitosterol and lanosterol, was found highest in pods(0.23mg/gdw) and 0.24mg/gdw) respectively, whereas highest level of campersterol was found in leaves(0.24mg/gdw). The isolated phytosterols were effective against all test bacteria and fungi. β-sitosterol was more active against fungi and bacteria and their MIC value was recorded 2 X 103 mg/disc while the MIC value of 3X 103 mg/disc was recorded for lanosterol, Campersterol and Stigmasterol.

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