||The main objective of the study was to determine of practices of sterilization techniques at private dental clinics of Karachi, Pakistan. 150 dental clinics of the town were selected, owned and run by qualified dentists. The purpose of the study was explained to the owners /practitioners verbal consent was taken. Author designed the questionnaire by close ended questions in order to get information for sterilization method and infection control. The survey was completed in two weeks while five house officers of Karachi Medical & Dental College participated to in filling of questionnaire. The sample size of the study was 150 with 95% confidence interval. Sample selection criteria: (Inclusion) Dentists, Dental hygienists and Dental assistants who are responsible or oversee the sterilization procedure from dental clinics of District Central Karachi. Exclusion: Reception staff, dental technicians. The result shows that 121 (81%) out of 150 clinics used autoclave as the method of sterilization and 15(10%) of the clinics used dry heat as the method of sterilization. While 14(9.3%) still employ the boiling and chemical as method of sterilization. The result shows that the 65.3% were the owner of the clinic and 34.7 % were dentist among the respondents. It is worth noting that 115(76.7) were female and 35(23.3%) male were among respondent. Among the dentist who answered the questioner 79.3% were BDS and 20.7% were postgraduates. From the results of the study it is quite evident that the Infection control measures taken in dental clinics were quite satisfactory. It is worth noting that almost 81% of the clinics used autoclaving method of sterilization. Internationally, the method used for sterilization is autoclaving for the sterilization of instruments in the private dental clinics. Some of the dental clinics used, chemical and dry heat sterilizer for the sterilizations of instruments. However, PMDC (Pakistan Medical & Dental Council) or any other government organization did not set any legal bindings for the quality and standards of dental practice in Pakistan.