||This study is an attempt to determine the genetic diversity and relationships among fourteen local isolate isolated
from patients with Aspergillosis (Aspergillus fumigatus) by using the simple sequence repeat polymerase chain
reaction (ISSR-PCR). Twelve universal primers used in this study produced 94 bands across fourteen isolates. Of
these bands, 67 bands or 71.2% were polymorphic. The size of the amplified bands ranged between 100-2,000 bp.
The genetic polymorphism value of each primer was determined and ranged between 33-100%. In terms of unique
banding patterns, determine the finger print for six isolates the most characteristic banding pattern was for the
(AFU1,AFU2,AFU3,AFU4,AFU8 and AFU14) with primer (RP F-16 , RP I-06, RP F-16, RP X-01, RP X-01and
OP A-06). Genetic distances ranged from 0.12419 to 0.64404 among A. fumigatus isolates. Cluster analyses were
performed to construct a dendrogram among studied A. fumigatus isolates.The cluster analysis places most of the
A.fumigatus isolates isolated from patient come from yhe same area into a close relation (subcluster) showing a high
level of genetic relatedness and were distinct from isolates from another area (the other subcluster). Interestingly, a
number of isolates originating from the same sources did form well defined groups, indicating association between
the ISSR patterns and the geographic origin of the isolates. The information generated from this study can be used in
the future for controlling of Aspergillosis programs.