||The present study was undertaken to investigate anti-inflammatory, thrombolytic and cytotoxic activities of
methanol extracts of Lablab niger leaves and its different fractions. In-vitro anti-inflammatory activity of the L.
niger leaves was evaluated by heat and hypotonic solution induced membrane stabilization method. Thrombolytic
activity was evaluated using blood clot lysis model and cytotoxicity test was carried out by brine shrimp lethality
bioassay. The crude methanolic extract and all tested fractions of L. niger leaves significantly (p< 0.005) protected
erythrocyte membrane lysis comparable to standard aspirin, demonstrating its strong anti-inflammatory activity. In
thrombolytic study, the crude methanolic extract and its different fractions demonstrated moderate to strong
thrombolytic activity (25.62-40.88% lysis of human blood clot), in comparison to 66.75 % clot lysis by standard
streptokinase. In the brine shrimp lethality bioassay, methanolic extract and its different fractions of L. niger leaves
showed significant lethality with LC50 =value of 1.171-8.15 μg/mL, compared with vincristine sulphate (LC50=0.451
μg/mL). These finding indicate that L. niger leaves could be a potential source of natural anti-inflammatory,
thrombolytic and cytotoxic agents.