||A specific, sensitive and rapid RP-HPLC method has been developed for the determination of Tirofiban in serum.
The chromatographic separation was realized using reverse phase LiChrospher®
100 RP-18 column (4.0 mm × 250
mm, 5 μm) and mobile phase consisting the mixture of 0.1 M KH2PO4 (pH 5.2, adjusted with 1.0 N sodium
hydroxide solution) and acetonitrile, with the ratio of 70:30% (v/v) and flow rate of 1.0 ml/min. The detection was
carried out at 274 nm. The response was linear over the range of 0.03 – 0.18 mgmL-1
in mobile phase and serum
samples. The limit of detection (LOD) for Tirofiban was 1.84, 13.8 and 14.6 μg mL-1 in methanol, spiked rat serum
and spiked human serum, respectively. The described method can be quickly and routinely applied, without any
interference from endogenous substances, for therapeutic monitoring of levels of Tirofiban in the serum samples.