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Title : PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL EVALUATION OF N-HEXANE EXTRACT OF GARCINIA KOLA HECKEL [BITTER KOLA] SEED
Authors : Uzondu Akueyinwa Lovet Esther, Utoh-Nedosa Uchechukwu Anastasia and Anowi Chinedu Fredrick
Abstract : The patented flavonoid extract of Garcinia kola seeds for the treatment of liver disorders has been shown to have anti-oxidant and scavenging properties. This study, therefore, evaluated the phytochemical composition of Garcinia kola seed and antimicrobial activity of the N-hexane extract of Garcinia kola seed against Pseudomonas aeruginosa; Staphylococcus aureus; Bacillus subtilis; Escherichia coli; Klebsiella pneumoniae and Candida albicans. The zones of inhibition of the N-hexane extract concentrations (100mg, 50mg and 25mg) against the growth of the organisms were determined by the cup-plate agar method. A Marcfarland Standard of each of the bacteria was used to seed sterile molten nutrient agar medium maintained at 450 C and Sabourand dextrose agar plate was seeded with Candida albicans. Results of phytochemical screening tests showed that Garcinia kola seed powder contained starch, protein, glycosides, flavonoids, tannin, saponins, sterols and triterpenoids. The antibacterial activity of 100mg extract was highest and was in the order: action on Bacillus subtilis > Pseudomonas aeruginosae >Staphylococcus aureus and Klebsiella pneumoniae with zones of inhibition of 10.66mm, 9.66mm, 9mm, 9mm, respectively while that of the 50mg concentration was of the order: Bacillus subtilis> Pseudomonas aeruginosa and Staphylococcus aureus >> Krebsiella pneumoniae with zones of inhibition of 8.33mm, 8. 33mm, 8.33mm and 4.66mm, respectively. The 100mg, 50mg and 25mg concentrations of the extract were all poorly inhibitory to Candida albicans and were resisted by Eshcerichia coli. The triterpenoids found in the extract (possibly in conjunction with the glycosides) are suggested to be responsible for the anti-bacterial and anti-fungal activity of the N-hexane extract of Garcina kola seed obtained in this study.

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