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Title : HPLC PROFILING OF POLYPHENOLIC COMPOUNDS AND FREE RADICAL ASSAY OF THE ETHANOLIC EXTRACT OF ANNONA RETICULATA GROWING IN BANGLADESH
Authors : Ismet Ara Jahan, Hemayet Hossain, Shaikh Emdadur Rahman, Shirin Sultana, Proity Nayeeb Akbar, Sabina Yeasmin, Tanzir Ahmed Khan, Md. Mahfuzur Rahman
Abstract : The present study investigates the HPLC profiling of phenolic compounds, and antioxidant activities of Annona reticulata. Through HPLC assay, high levels of rutin hydrate (603.88 mg/100 g extract) and moderate amount of catechin, epicatechin, and p-coumeric acid were determined (99.00, 78.19, 85.38 mg/100 g extract, respectively). Some amount of vanillic acid, ellagic acid, and quercetin were also detected (19.80, 31.56, 8.83 mg/100 g extract, respectively). Antioxidant activity tests such as, ABTS radical scavenging activity, reducing power, total antioxidant capacity, total phenolic and flavanoid content determination, were also performed. At 250 μg/ml, the percentage inhibition value of Annona reticulata for ABTS scavenging was 99.93 μg/ml, and the maximum absorbance for reducing power assay was 0.195. The total antioxidant capacity, and phenolic and flavonoid contents were calculated to be 419.20 mg of ascorbic acid/g, 120.07 mg/g of gallic acid, and 90.05 mg/g of quercetin equivalent, respectively. These results suggested that polyphenolic compounds in Annona reticulata might be responsible for their antioxidant activities.

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