||The current study was aimed to evaluate the antibacterial activity of different concentrations of aqueous and non-aqueous extracts of Carica papaya seeds. Dried and grinded papaya seeds (5g), respectively mixed with 95ml of extraction solvent (water, methanol, acetone and ether) for 24 hours were used for this study. Extraction was done by maceration and Soxhlet method. The extract was then decanted and filtered through a Whatman filter paper. Antimicrobial activities of the each extract were determined using agar well diffusion method using fresh clinical isolates of Escherichia coli and Staphylococcus aureus and their MIC and zone of inhibition were determined. The results were evaluated statistically using paired sample t-test. Aqueous extract of Carica papaya exhibits greater antimicrobial activities against the clinical isolates of Escherichia coli and Staphylococcus aureus as compared to the non-aqueous plant extracts. MIC of aqueous extract against Escherichia coli was 10-3mg/mL, whereas methanolic extracts shows 10-4mg/mL MIC. Similarly MIC of aqueous extract against Staphylococcus aureus was 10-4mg/mL as compared to MIC of methanolic extract 10-6mg/mL. There was statistically significant difference between the zone of inhibition of aqueous extracts of the plant material against Staphylococcus aureus and Escherichia coli (P < 0.001). Preliminary phytochemical analyses reveal that the extracts contain alkaloids, tannins, saponins and phenols. Carica papaya may be used for the treatment of infection caused by Escherichia coli and Staphylococcus aureus.