||Inadequate knowledge of diabetes among health professionals can jeopardise the management of diabetes among patients. A Cross-sectional study was conducted to assess the knowledge of Diabetes among Pharmacists and Nurses in a Nigerian tertiary Health care centre. Knowledge of the effects of exercise on blood glucose level had a positive score of 92.3% among Pharmacists. About 100% of Pharmacists had knowledge of Diabetes and its complications. The causes of low blood glucose had a frequency score of about 96.2% among pharmacist. The result in relation to diabetes as a disease and its complications showed that more than 50% of Nurses exhibited sound knowledge. The knowledge of the complications of diabetes had also a good frequency score of about 88.1% and the causes of low blood glucose had the highest score of about 89.1% among Nurses. Diabetes is a serious and growing health problem in the world and broad diabetes knowledge is necessary to ensure excellent care of this growing population.