||To evaluate the hepatoprotective activity of crude ethanolic leaf extract of Alstonia macrophylla G. Don in Paracetamol induced hepatotoxicity in Sprague Dawley rats. Hepatotoxicity was established by induction of Paracetamol 500mg/kg leading to elevated liver markers, Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Alanine phosphatase (ALP), and decreased Albumin levels. Single dose administration with Alstonia macrophylla crude extract (AmCE) on a 7-day duration treatment, resulted in decreased AST, ALT, and ALP and increased Albumin levels. Specifically the AmCE 600mg/kg resulted to significant effects at (p<0.01). Similarly, depleted levels of Glutathione, and Catalase levels were increased (p<0.003), (p<0.000), respectively. While Malondialdehyde – a measure of lipid peroxidation, was significantly decreased (p<0.000). Among the treatments, AmCE 600mg dose showed comparable effect with the standard drug N-acetylcysteine. This study demonstrated the hepatoprotective activity of the crude ethanolic leaf extract of Alstonia macrophylla.