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Title : A VALIDATED LIQUID CHROMATOGRAPHIC ESTIMATION OF DROTAVERINE IN HUMAN PLASMA
Authors : B.Jyothirmai, B. Syama Sundar, Tata Santosh
Abstract : The present work describes a rapid, selective and accurate reverse phase HPLC method for the determination of Drotaverine using Paracetamol as internal standard. The extraction process involved was protein precipitation technique.  Drotaverine and internal standard were eluted under isocratic mode. Best resolution of the drug and internal standard was achieved with a mobile phase of 10mM potassium dihydrogen phosphate buffer and methanol in the ratio of 25:75v/v.  The recovery of Drotaverine 76.41% and of Paracetamol 84.49%.

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