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Title : VALIDATED RP-HPLC METHOD FOR THE ESTIMATION OF PANTOPRAZOLE IN PURE AND TABLET DOSAGE FORM
Authors : Madhukara B M, Jose Gnana Babu C, Thamizh Mani T.
Abstract : A simple, specific, accurate, precise and sensitive reverse phase high performance liquid chromatographic method has been developed for the quantitation of Pantoprazole in both pure and tablet dosage forms. A Waters XTerra RP18(C-18), 5 µm column having 250×4.6 mm i.d. in isocratic mode with mobile phase containing Acetonitrile: Methanol: Water (40:40:20 v/v/v). The flow rate was 1.0 ml/min and the effluents were monitored at 295 nm. The retention time was 2.9 min. The linearity was in the range of 2-10 µg/ml. This method was validated for linearity, precision, specificity, limit of detection, limit of quantitation, accuracy, ruggedness and robustness. Statistical analysis proves that the method is reproducible and selective for the estimation of the said drug.

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