||Urinary tract infections are the most common bacterial infection in pregnancy. An untreated Urinary tract infection can lead to increased risk of maternal and neonatal morbidity and mortality. Objective of the study was to determine the prevalence of Urinary tract infection in pregnant women, causative organism and antibiotic sensitivity pattern of isolated uropathogens. The study was conducted in the Gynaecology department of a single speciality private hospital for a study period of 6 months and sample size of 160 patients. Urine samples of the sample population were undergone for microscopic and culture and sensitivity tests. Majority of sample population affected Urinary tract infection were between an age group of 20-29 years (58%) and the incidence of infection was common in third (51%) and second (32%) trimesters. Escherichia coli [102(64%)] was the commonest bacterial pathogen isolated and others were Klebsiella pneumonia [29(18%)], Pseudomonas aeruginosa [13(8%)], Staphylococcus aureus [9(6%)] and Proteus mirabilis [7(4%)] . Levofloxacin had the highest overall antibiotic sensitivity of 83% followed by Ofloxacin (80%) ,Ceftriaxone (77%),Ciprofloxacin(74%), Amikacin (67.5%), Gentamycin (46%), Nitrofurantoin (38%), Amoxicillin- clavulanate (26%), Amoxicillin (15%), Doxycyclin (7%), Clotrimoxazole (7%).