||A simple, precise, rapid and accurate reverse phase HPLC method was developed for the estimation of Azithromycin in tablet dosage form.Xterra RP 18 (250x4.6mm, 5m particle size), with mobile phase consisting of ortho-phosphori acid:methanol 70:30 V/V was used. The flow rate 1.0 ml/min and the effluents were monitored at 205 nm.The retention time and Recovery time was 12 minutes. The detector response was linear in the concentration of 25-275 mg/mL.The respective linear regression equation being Y= 1228.302 x+5230.5524. The limit of detection and limit of quantification was 12.5mcg and 37.5mcg/ml respectively. The percentage assay of Azithromycin was 98.0%. The method was validated by determining its accuracy, precision and system suitability.The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Azithromycin in bulk drug and in its pharmaceutical dosage form.