View                 Download                 XML
Title : SIMULTANEOUS QUANTIFICATION OF RISPERIDONE AND ESCITALOPRAM IN HUMAN PLASMA BY LC-MS/MS: APPLICATION TO A PHARMACOKINETIC STUDY
Authors : Pallavi Alegete, Prasad Kancherla, Saeed S. Albaseer, and Sathyanarayana Boodida
Abstract : A solid phase extraction method was developed for the simultaneous quantification of risperidone and escitalopram in human plasma by a suitable ultra-performance liquid chromatographic / tandem mass spectrometric assay (LC-MS/MS) .Resperidone-D4 was used as an internal standard. The method involves simple isocratic chromatographic conditions at a flow rate of 0.400 mL min-1. Samples were separated on X-terra RP8 column (50 mm × 4.6, 5µm) in a shorter run time of only 2.0 min using a mobile phase mixture of 95:5 v/v (acetonitrile : ammonium acetate) buffer 5mM, pH 5.0±0.05). Calibration plots were linear (r2 0.99) over the concentration range of 0.050 to 26 ng mL-1 for risperidone and 0.100 to 51 ng mL-1 for escitalopram. The overall recoveries for risperidone and escitalopram were 76.88% and 81.14%, respectively. Precision was 2.9%, 5.07% (intra-day) and 4.13%, 6.43% (inter-day) for risperidone and escitalopram, respectively. The validated method was successfully applied to a pharmacokinetic study of human plasma.

A PHP Error was encountered

Severity: Notice

Message: Undefined index: HTTP_REFERER

Filename: views/issue_title.php

Line Number: 65

Backtrace:

File: /var/www/html/pharmascholars-com/app_psl/views/issue_title.php
Line: 65
Function: _error_handler

File: /var/www/html/pharmascholars-com/app_psl/controllers/PSL.php
Line: 144
Function: view

File: /var/www/html/pharmascholars-com/index.php
Line: 293
Function: require_once

">Back