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Title EVALUATION OF ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF THE BARK OF HOLARRHENA PUBESCENS, ITS FRACTIONS AND CONESSINE
Authors *Bina S. Siddiqui, Syed Tahir Ali, Saima Tauseef, Saira Kamal, Ghazala H. Rizwani, Sabira Begum, and Aqeel
Description The present study was carried out to investigate the antioxidant effect of the methanolic extract, its alkaloidal and non-alkaloidal fractions along with petroleum ether soluble, ether soluble and ethyl acetate soluble sub-fractions of non-alkaloidal part of the bark of Holarrhena pubescens. The pure compound conessine was also tested. The activity was determined by using DPPH free radical scavenging assay at 500 μg/mL for the extract and the fractions, and at 200 μg/mL for conessine and the standard (ascorbic acid). The non-alkaloidal fraction was found to be more active (63% inhibition; EC50 = 250 μg / mL) than the alkaloidal fraction (16% inhibition) and its polar ethyl acetate soluble fraction was found to be most active (70% inhibition; EC50 = 250 μg / mL). Conessine was non-active at the concentration used.

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