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Title ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF LOSARTAN POTASSIUM AND ENALAPRIL MALEATE IN TABLET DOSAGE FORM BY RP-HPLC
Authors Sindu Yada, A. Ajitha, V. Uma Maheshwara Rao
Description A simple, specific and accurate reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of Losartan potassium and Enalapril maleate in tablet dosage form.  The using Agilent C8 (4.6 x 150mm, 3.5mm) column in Isocratic mode, in mobile phase containing Acetonitrile phosphate buffer (50:50) and adjusted Ph. 6.5with ortho phosphoric acid, the flow rate was 2ml/min. The detection was carried out at wavelength 256nm. The retention times of Losartan potassium and Enalapril maleate were found to be 3.537 and 2.109 min respectively. The linearity for Losartan potassium and Enalapril maleate were in the range of 25-125µg/ml and 5-25µg/ml  respectively .The recoveries of Losartan potassium and Enalapril maleate were found to be 99.7%and99.2%,respectively. The LOD values for  and LOQ values are found to be within acceptance criteria. The LOD values were found to be 2.97 and 2.95 for Losartan potassium and Enalapril maleate respectively and LOQ values were found to be 9.91 and 9.95 for Losartan potassium and Enalapril maleate respectively.  The proposed method was validated and successfully applied for the estimation of Losartan potassium and Enalapril maleate in combined tablet dosage forms.

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