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Title DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF ATENOLOL AND AMLODIPINE IN BULK AND TABLET DOSAGE FORM
Authors Akki Srivani, Sujitha Hazari and Swathi Malichetti
Description A new precise, accurate, reliable validated method for the determination of Atenolol and Amlodipine has been developed by using reverse phase high performance liquid chromatography (RP-HPLC) in pharmaceutical dosage form. Chromatographic separation was carried out by using mobile phase 0.02M Potassium dehydrogenate phosphate: acetonitrile (62:38v/v, PH-3.56 adjusted with Orthophosphoric acid) on Hypersil, BDS 150 x 4.6 mm, 5 at a flow rate 0.8ml/min with UV detection at 238nm.The retention times for Atenolol and Amlodipine were 1.998 and 6.093 min respectively and both drugs showed good linearity in the range of 250-750 µg/ml and 25-75 µg/ml. The proposed method has been successfully applied to pharmaceutical formulation and was validated according to ICH guidelines and method showed good precision with percentage relative standard deviation less than 2%. The percentage recovery for Atenolol and Amlodipine was found between 99.06-100.94% and 99.12-100.95% respectively indicating the proposed method was accurate and precise.

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