Abstract

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Title QUANTIFICATION OF OLMESARTAN AND ROSUVASTATIN BY STABILITY INDICATING RP-HPLC IN PHARMACEUTICAL DOSAGE FORM
Authors A.Nagavalli, M. Prasada Rao
Description

The objective of the study is to develop an economical and simple and validated method for the simultaneous estimation of the olmesartan and rosuvastatin by RP-HPLC technique. In this method the 10µl of standard working solution containing both olmesartan and rosuvastatin was injected in to the mobile phase line composing of 45B and 55A, pumped through the column Altima 150mm length column containing particle of size 5µ with a flow rate of 1ml/min. the temperature of the column was maintained at 30°C. Both the drugs have their optimum absorbance at 241nm wavelength. The method was optimized based on the all system suitable parameters passed their limits as per ICH guidelines. The retention time found was 2.2min of olmesartan and 3.0min of rosuvastatin and the resolution between the peaks was 4.8. This method was set for validation as per ICH guidelines and observed to be very specific without any interference by the constituents of formulations and diluents. The method was precisied and %RSD found to be 0.56 of olmesartan and 0.42 of rosuvastatin. % recover of olmesartan was 100.09% and 99.79% for rosuvastatin. Linearity was performed with six concentrations and response was observed to be linear to the concentration. Correlation coefficient obtained was 0.999. The developed method was robust as the %RSD was within the range and without effecting system suitability parameters. The developed method passed all the parameters of the validation, the run time was decreased effectively so the method was very simple and economical that can be used in the regular analysis of the Olmesartan and rosuvastatin in marketed formulation.       

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