Abstract

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Title RP-HPLC METHOD FOR THE DETERMINATION OF DOMPERIDONE AND ESOMEPRAZOLE IN COMBINED DOSAGE FORM
Authors M. Akiful Haque*, S. Hasan Amrohi, A. Manikanta Kumar, Dibyalochan Mohanty and Prakash V Diwan
Description <span style="font-size: 10pt; font-family: &#39;Times New Roman&#39;, serif">A simple, specific and accurate reverse phase high performance liquid chromatographic method was developed for the quantitative determination of </span><span style="font-size: 10pt; font-family: &#39;Times New Roman&#39;, serif">Lornoxicam</span><span style="font-size: 10pt; font-family: &#39;Times New Roman&#39;, serif; letter-spacing: -0.05pt"> </span><span style="font-size: 10pt; font-family: &#39;Times New Roman&#39;, serif">in bulk drug and formulation. </span><span style="font-size: 10pt; font-family: &#39;Times New Roman&#39;, serif">T<span style="letter-spacing: -0.05pt">h</span>e<span style="letter-spacing: 0.25pt"> </span>d<span style="letter-spacing: -0.05pt">e</span><span style="letter-spacing: -0.1pt">v</span><span style="letter-spacing: 0.1pt">e</span><span style="letter-spacing: -0.05pt">l</span>o<span style="letter-spacing: -0.05pt">p</span>ed<span style="letter-spacing: 1.1pt"> </span><span style="letter-spacing: 0.05pt">m</span>ethod<span style="letter-spacing: 0.7pt"> </span>co<span style="letter-spacing: -0.05pt">n</span>s<span style="letter-spacing: -0.05pt">i</span>s<span style="letter-spacing: 0.05pt">ts</span><span style="letter-spacing: -0.05pt"> of</span><span style="letter-spacing: 0.05pt"> m</span>o<span style="letter-spacing: -0.05pt">bil</span>e p<span style="letter-spacing: -0.05pt">h</span>ase, </span><span style="font-size: 10pt; font-family: &#39;Times New Roman&#39;, serif">ammonium dihydrogen phosphate Buffer: acetonitrile (50:50) with isocratic </span><span style="font-size: 10pt; font-family: &#39;Times New Roman&#39;, serif">pr<span style="letter-spacing: -0.1pt">o</span><span style="letter-spacing: 0.1pt">g</span><span style="letter-spacing: 0.05pt">r</span><span style="letter-spacing: -0.15pt">a</span><span style="letter-spacing: 0.05pt">mm</span><span style="letter-spacing: -0.05pt">i</span><span style="letter-spacing: -0.15pt">n</span><span style="letter-spacing: 0.1pt">g</span>, </span><span style="font-size: 10pt; font-family: &#39;Times New Roman&#39;, serif">Hypersil BDS C<sub>18</sub>, 250&times;4.6mm, 5&micro;m</span><span style="font-size: 10pt; font-family: &#39;Times New Roman&#39;, serif"> co<span style="letter-spacing: -0.05pt">l</span>umn<span style="letter-spacing: 1.45pt"> </span>as<span style="letter-spacing: 0.8pt"> </span>s<span style="letter-spacing: 0.05pt">t</span>ati<span style="letter-spacing: -0.05pt">o</span>n<span style="letter-spacing: -0.05pt">a</span><span style="letter-spacing: 0.05pt">r</span>y<span style="letter-spacing: 1.7pt"> </span>p<span style="letter-spacing: -0.05pt">h</span>ase<span style="letter-spacing: 1.35pt"> </span><span style="letter-spacing: -0.05pt">wi</span><span style="letter-spacing: 0.05pt">t</span>h<span style="letter-spacing: 1.05pt"> </span>a<span style="letter-spacing: 0.65pt"> </span><span style="letter-spacing: 0.15pt">f</span><span style="letter-spacing: -0.05pt">l</span>ow <span style="letter-spacing: 0.05pt">r</span>ate<span style="letter-spacing: 0.9pt"> </span><span style="letter-spacing: -0.15pt">o</span>f<span style="letter-spacing: 0.8pt"> </span><span style="letter-spacing: -0.15pt">1</span><span style="letter-spacing: 0.05pt">.</span>0<span style="letter-spacing: 0.9pt"> </span><span style="letter-spacing: 0.05pt">m</span><span style="letter-spacing: -0.05pt">L/</span><span style="letter-spacing: 0.05pt">m</span><span style="letter-spacing: -0.05pt">i</span>n.<span style="letter-spacing: 1.85pt"> </span><span style="letter-spacing: -0.05pt">Pr</span>o<span style="letter-spacing: -0.05pt">p</span>os<span style="letter-spacing: -0.05pt">e</span>d<span style="letter-spacing: 1.7pt"> </span><span style="letter-spacing: 0.05pt">m</span>ethod<span style="letter-spacing: 1.35pt"> </span><span style="letter-spacing: -0.15pt">w</span>as <span style="letter-spacing: 0.9pt">&nbsp;</span><span style="letter-spacing: 0.15pt">f</span><span style="letter-spacing: 0.1pt">o</span><span style="letter-spacing: -0.15pt">u</span>nd<span style="letter-spacing: 1.15pt"> </span><span style="letter-spacing: 0.05pt">t</span>o<span style="letter-spacing: 0.65pt"> </span>be<span style="letter-spacing: 0.7pt"> </span><span style="letter-spacing: -0.05pt">li</span>n<span style="letter-spacing: -0.05pt">e</span>ar<span style="letter-spacing: 1.15pt"> </span><span style="letter-spacing: -0.05pt">i</span>n<span style="letter-spacing: 0.6pt"> </span><span style="letter-spacing: 0.05pt">t</span>he<span style="letter-spacing: 0.8pt"> </span>co<span style="letter-spacing: -0.05pt">n</span>ce<span style="letter-spacing: -0.05pt">n</span><span style="letter-spacing: 0.05pt">tr</span><span style="letter-spacing: -0.15pt">a</span><span style="letter-spacing: 0.05pt">t</span><span style="letter-spacing: -0.05pt">i</span>on<span style="letter-spacing: 2.2pt"> </span><span style="letter-spacing: 0.05pt">r</span>a<span style="letter-spacing: -0.15pt">n</span><span style="letter-spacing: 0.1pt">g</span>e<span style="letter-spacing: 1.1pt"> </span><span style="letter-spacing: -0.15pt">o</span>f </span><span style="font-size: 10pt; font-family: &#39;Times New Roman&#39;, serif">5 &ndash; 30 </span><span style="font-size: 10pt; font-family: &#39;Times New Roman&#39;, serif; letter-spacing: -0.1pt">&micro;g/mL</span><span style="font-size: 10pt; font-family: &#39;Times New Roman&#39;, serif">, <span style="letter-spacing: 0.45pt">the</span> cor<span style="letter-spacing: 0.05pt">r</span>e<span style="letter-spacing: -0.05pt">l</span>ati<span style="letter-spacing: -0.05pt">o</span>n coefficient <span style="letter-spacing: 0.05pt">was</span><span style="letter-spacing: 1.75pt"> </span><span style="letter-spacing: 0.15pt">f</span>o<span style="letter-spacing: -0.05pt">u</span>nd<span style="letter-spacing: 2.1pt"> </span><span style="letter-spacing: 0.05pt">t</span>o<span style="letter-spacing: 1.65pt"> </span>be<span style="letter-spacing: 1.55pt"> </span>0.99<span style="letter-spacing: -0.15pt">9</span>.<span style="letter-spacing: 2.25pt"> </span></span><span style="font-size: 10pt; font-family: &#39;Times New Roman&#39;, serif">System suitability parameters were studied by injecting the standard solution five times and results were well under the acceptance criteria</span><span style="font-size: 10pt; font-family: &#39;Times New Roman&#39;, serif">,<span style="letter-spacing: 0.85pt"> </span><span style="letter-spacing: 0.1pt">the</span><span style="letter-spacing: 2.35pt"> </span>propos<span style="letter-spacing: -0.05pt">e</span>d&nbsp; <span style="letter-spacing: 0.15pt">&nbsp;</span><span style="letter-spacing: 0.05pt">m</span>e<span style="letter-spacing: -0.1pt">t</span>h<span style="letter-spacing: -0.05pt">o</span>d is<span style="letter-spacing: 2.05pt"> </span><span style="letter-spacing: 0.15pt">f</span>o<span style="letter-spacing: -0.05pt">u</span>nd<span style="letter-spacing: 2.55pt"> </span><span style="letter-spacing: 0.05pt">t</span>o<span style="letter-spacing: 2.05pt"> </span>be se<span style="letter-spacing: -0.05pt">n</span>s<span style="letter-spacing: -0.05pt">i</span><span style="letter-spacing: 0.05pt">t</span><span style="letter-spacing: -0.05pt">i</span><span style="letter-spacing: -0.1pt">v</span>e,<span style="letter-spacing: 1.15pt"> </span><span style="letter-spacing: 0.05pt">r</span>a<span style="letter-spacing: -0.05pt">pi</span><span style="letter-spacing: -0.15pt">d</span>,<span style="letter-spacing: 0.9pt"> </span><span style="letter-spacing: 0.05pt">r</span>e<span style="letter-spacing: -0.05pt">p</span><span style="letter-spacing: 0.05pt">r</span>o<span style="letter-spacing: -0.05pt">d</span>uc<span style="letter-spacing: -0.05pt">i</span>b<span style="letter-spacing: -0.05pt">l</span>e,<span style="letter-spacing: 1.85pt"> </span>a<span style="letter-spacing: -0.05pt">n</span>d<span style="letter-spacing: 1.95pt"> </span>acc<span style="letter-spacing: -0.05pt">u</span><span style="letter-spacing: 0.05pt">r</span><span style="letter-spacing: -0.15pt">a</span><span style="letter-spacing: 0.05pt">t</span>e<span style="letter-spacing: 0.5pt">.</span></span>

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