Country-wise Listing - France

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S.NO Title & Authors Name page
1
DEVELOPMENT OF A HPLC METHOD FOR THE DETERMINATION OF ENROFLOXACIN AND CIPROFLOXACIN IN REPTILE PLASMA AFTER TRANSDERMAL DELIVERY
Iga Czyz, Jean-Paul Salvi, Alban Ducrotte, Plamen Kirilov, Sebastien Perrot, Charly Pignon, Fabrice Pirot, Francoise Falson, Roselyne Boulieu
 Abstract                  View                 Download                 XML
A rapid and simple high-performance liquid chromatography method using diode array detection for determination of enrofloxacin and ciprofloxacin in snake’s plasma was developed. Several snakes were treated transdermally with a single dose of enrofloxacin emulsions corresponding to an antibiotic dose of 5 mg/kg. Enrofloxacin and ciprofloxacin were analyzed using a core-shell silica particle stationary phase (Kinetex  RP–C18, 150 × 4.6 mm, particle size 5 µm) and 0.002 M phosphoric acid/acetonitrile (83:17, v/v) as mobile phase. Calibration curves were linear over the concentration range of 2–100 µM and 3–100 µM. The intra-day and inter-day coefficient of variations were below or equal to 10% for both compounds. Limits of quantification for enrofloxacin and ciprofloxacin were 2 µM and 3 µM respectively. Sample treatment procedure consisted of deproteinization with perchloric acid. The described HPLC method using core-shell silica particles results in better resolution, higher sensitivity and low back pressure.

571-576
2
DEVELOPMENT OF A SIMPLE HPLC METHOD USING CORE-SHELL PARTICLES COLUMN FOR QUANTIFICATION OF IR3535 IN INSECT REPELLENT LIQUID FORMULATIONS.
Sinh Thai Nguyen, Jean-Paul Salvi, Elodie Gilbert, Celine Serre, Fabrice Pirot, Roselyne Boulieu
 Abstract                  View                 Download                 XML
A simple, fast, precise and sensitive method for the quantification of IR3535, an insect repellent used in topical formulations, was developed by reversed-phase high performance liquid chromatography. The separation was carried out by using a core-shell silica particles column (Kinetex  C18, 5??m, 4.6 mm x 150 mm) with a mobile phase consisting of 0.1% formic acid and methanol (40 : 60 v/v) in an isocratic mode at 0.5 ml/min and UV detection at 220 nm. The calibration curve was linear from 10 to 400 ??M, with regression coefficient r2 = 0.9999. Limit of detection (LOD) and Limit of quantification (LOQ) were found to be 1 and 10 ??M, respectively. Precision and accuracy fulfilled the acceptance criteria. This method was validated and may be routinely used for quantitative analysis of IR3535 from various topical insect repellent liquid formulations.
9-14