Country-wise Listing - Morocco

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S.NO Title & Authors Name page
1
EVALUATION OF ANTI-INFLAMMATORY ACTIVITY OF MATRICARIA CHAMOMILLA L. ESSENTIAL OIL IN VIVO FROM MOROCCO
Ghizlane Hajjaj, Aziz Bahlouli, Mouna Tajani, Yahia Cherrah and Amina Zellou
 Abstract                  View                 Download                 XML
The present work was carried out to evaluate the anti-inflammatory activity of Matricaria chamomilla L. essential oil in rodents. The flowers of Matricaria chamomilla L. was subjected to extraction by hydrodistillation and the chemical composition of the essential oil was assessed through gas chromatography coupled to mass spectrometry (GC/MS). The effect of the essential oil against acute inflammation was studied using carrageenan and experimental trauma-induced hind paw edema in rats at different doses (100, 200 mg/kg p.o.) and compared with standard drug (indomethacin) and with control group. Matricaria chamomilla L. essential oil contained Chamazulene (25.21 %), Cis-beta-farnesene (12.51 %), Eucalyptol (9.19 %), Coumarin (7.72 %), Galaxolide (6.28 %) and Camphor (4.3 %) this essential oil produced significant anti-inflammatory effect (P<0.001) at all doses studied in both models when compared with control group. This study revealed that M. chamomilla L. essential oil has a potent anti-inflammatory activity. This effect, at least in part, depend upon the chemical composition properties of the essential oil of this medicinal plant from Morocco.
31-36
2
BIOLOGICAL INVESTIGATIONS OF ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES OF THYMUS SATUREIOIDES COLLECTED IN TAFILALET REGION, SOUTH-EAST OF MOROCCO
Mhamed Ramchoun, Khalid Sellam, Hicham Harnafi, Chakib Alem, Mohamed Benlyas, Lhoussaine EL Rhaffari, Souliman Amrani
 Abstract                  View                 Download                 XML
The present study was carried out to investigate the antioxidant an antimicrobial effect of different extracts of Thymus satureoïdes. The FRAP assay shows that the antioxidant activity was between 07.03±0.29 and 117.51±6.46 mmol Trolox/g of extract. The IC50 values from different extracts of Thymus satureoides were between 0.41±0.005 and 43.89±2.46 mg/mL of extract. The different extracts were also, demonstrated the important significantly (p<0.001) reduction of the malondialdehyde. The total polyphenols extract was particularly found to possess stronger antimicrobial activity.
339-346
3
ONE POT SYNTHESIS, ANTITUMOR, ANTIBACTERIAL AND ANTIFUNGAL ACTIVITIES OF SOME SCHIFF BASE HETEROCYCLES
Smaail Radi, Said Tighadouini, Olivier Feron, Olivier Riant, Yahia Nasser Mabkhot, Salim S. Al-Showiman, Taibi Ben Hadda, Mohammed El-Youbi, Redouane Benabbes, Ennouamane Saalaoui
 Abstract                  View                 Download                 XML
A series of Schiff bases of furan and thiophen rings has been synthesized in one step, in good and excellent yields, using acetic acid as catalyst. The synthesized products were evaluated for their anticancer activity toward breast (MDA-MB231) and colorectal (LoVo) human cell lines cancers. The in vitro antibacterial and antifungal potential of the products were also determined using tree bacterial strains (Echerichia coli, Bacillus subtilis, and Micrococcus luteus) and fungal strains (Fusarium oxysporum f.sp albedinis FAO). Significant activity against breast cancer cell lines and Fungal strains was observed.
39-45
4
COMMUNICATION ON SANITARY CRISIS SITUATION: CASE OF PANDEMIC INFLUENZA A/H1N1 (2009)
Naoual Laaroussi, Soufiane Derraji, Abdelghani Drhimeur, Abdelaziz Barkia
 Abstract                  View                 Download                 XML
This study deals with the theme of communication in a health crisis. It was conducted at the university hospital Ibn Sina in Rabat (CHIS) and was undertaken to describe the communication implemented for the benefit of health professionals before, during and after the crisis of pandemic influenza A/H1N1 (2009). The problem started from an personal irritation and from a statement approved by stakeholders who are sharing the same irritation. The literature review has identified elements describing of the communication during the three phases of the evolution of a crisis on the scale of a pandemic which formed the conceptual framework used to develop different parts descriptive of this work. Data collection was conducted through a questionnaire survey and by semi-structured interviews. Quantitative Analysis of the results obtained clearly showed a lack in communication especially during the pre and post crisis phases. Nevertheless, several communication actions have been deployed by CHIS using even developed technological means (website…) to inform and educate its staff during the pandemic. However, the media were the main source of information for these health professionals. This leads to uncertainty and installation of rumors among them.
140-145
5
Investigation of essential oil of melissa officinalis for acute and sub-chronic oral toxicity
Amina Bounihi, Rachad Alnamer, Ghizlane Hajjaj, Yahia Cherrah, and Amina Zellou
 Abstract                  View                 Download                 XML
This study is aimed at determining the acute and Sub-chronic oral toxicity of Melissa officinalis essential oil and to serve as criteria to recommend the ethno pharmacological uses of the plant. In the acute toxicity test, oral administration of 300 and 2000mg/kg of the Melissa officinalis essential oil was evaluated in mice. Sub-chronic toxicity was studied by daily oral dose of 100 and 200mg/kg for three month. On day 0, 30, 60 and 90, blood samples collected from retro-orbital sinus of the eye rats were used for evaluation of serum biochemistry, hematology and histopathological examination of the heart, lungs, liver, kidney and spleen. The acute toxicity oral study revealed no observable signs of toxicity. In rats, the examination of signs, animal behavior and the changes in body and organs weights showed no abnormalities in the test groups as compared to the controls. There were no significant variations in the hematological parameters of both Melissa officinalis essential oil treated and untreated rats. However, biochemical parameters showed significant decrease in blood sugar. Melissa officinalis essential oil significantly (p<0.05) increased serum urea suggesting degenerative changes in the kidney. We can conclude that, Melissa officinalis essential oil is well tolerated in short term therapies but may have long term toxic effects on the kidney.
40-46
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SYNTHESIS AND PHARMACOLOGICAL EVALUATION OF SOME NEW PYRAZOLE DERIVATIVES
Khalid Karrouchi, Latifa Doudach, Laila Chemlal, Mohamed Karim, Jamal Taoufik, Yahia Cherrah, Smaail Radi, My El Abbes Faouzi and M’hammed Ansar
 Abstract                  View                 Download                 XML
A new series of pyrazole derivatives have been synthesized by the reaction of substituted pyrazole carbohydrazide and functionalized aromatic aldehydes. All the compounds have been characterized by IR, 1H-NMR and mass spectroscopy. The analgesic activity of the synthesized compound was assessed by tail flick method (for central action) in rat, acetic acid-induced writhing test (for peripheral action) in mice. The effect of the synthesized compounds N’-(2,4-dichlorobenzylidene)-5-methyl-1H-pyrazol-3-carbohydrazide (5a), N\'-(2,4-dichlorobenzylidene)-2-(3,5-dimethyl-1H-pyrazol-1-yl)acetohydrazide(9), N’-(4-dimethylaminobenzyli-dene)-5-phenyl-1H-pyrazol -3-carbohydrazide (5b) and N’-(2,4-dichlorobenzylidene) -5-phenyl-1H-pyrazol-3-carbohydrazide (5c) was also investigated in a battery of behavioural models in mice to assess their sedative effect. Among them, 5b was found more potent in comparison to 150 mg/kg of acetylsalicylic acid (ASA) with 44.83% of inhibition. In radiant heat tail-flick test the synthesized compounds 5a, 9, 5b and 5c produced 11.06%, 9.73%, 8.38% and 19.31% (p<0.01) elongation of tail flicking time 30 minutes after oral doses of 100 mg/Kg body weight respectively. Further compounds after 60 min, the inhibition of pain were 8.27%, 8.97%, 6% and 12.98% respectively. The synthesized compounds at doses of 50 mg/Kg body weight tested were insignificant when compared with the control. Furthermore the oral administration in mice of compounds at 50 and 100 mg / kg, induces strong sedative effect compared to reference substance Nesdonal and significantly reduced in both the reestablishment time and number of head dips during the traction and hole-board tests, in the rotarod test the pyrazole derivatives significantly reduced the motor coordination of the tested animals. From the results the pyrazole derivatives exhibited antinociceptive activity by central and peripheral mechanism(s) and possess potent sedative effect.
79-87
7
IN VITRO CYTOTOXICITY AND FREE RADICAL SCAVENGING ACTIVITY OF ETHANOLIC AND ALKALOIDIC EXTRACTS OF DELPHINIUM STAPHISAGRIA
Bouchra Faridi, Latifa Doudach, Rachad Alnamer, Moulay Abbas Faouzi, FatimaTahiri, Yahia Cherrah and Amina Zellou
 Abstract                  View                 Download                 XML
In this study, the in vitro cytotoxicity and antioxidant properties of the ethanolic and alkaloidic extract of Delphinium Staphisagria seeds were assessed. The antioxidant activity of the plant extracts and the standard was assessed on the basis of the radical scavenging effect of the stable 1, 1-diphenyl-2-picrylhydrazyl (DPPH)-free radical activity. The in vitro cytotoxicity was carried out by using the 3-(4,5- dimethylthiazol- 2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, against two animals cancer cell lines; Vero cell line, initiated from the kidney of a normal, adult African green Monkey and Neuro-2a (N2a) from a spontaneous tumor of a strain A albino mouse. The DPPH scavenging activity of both extracts was concentration-dependent (increasing from 15.62 &micro;g/ml to 500 &micro;g/ml), exhibited considerably (P&lt;0.05) DPPH radical-scavenging activity and was able to inhibit the formation of DPPH radicals with a percentage inhibition of 62.19 and 94 % respectively at the highest concentration. The results showed that the alkaloidic extract (1000-31.25 &micro;g/ml) of Delphinium Staphisagria possesses significant IC50 compared to the drug positive control on all cancer cell lines used. The lower IC50 represent the highest potency of a compound to inhibit the growth of cells and cause toxicity and death of cells. The result obtained in this work demonstrated a high activity at low alkaloids extracts doses (500&micro;g/ml). In conclusion, the results of the present study suggest that Delphinium Staphisagria seeds plant have various secondary metabolites and has good quantity of alkaloidic compounds (diterpenoid alkaloids). Plant has potent free radical scavenging activity. Detailed studies on chemical composition, isolation of active constituents and pharmacological evaluation are essential to characterize them as biological antioxidants. The present findings of this study support the view that Delphinium Staphisagria seeds are a promising source of potential antioxidant which can be used in treatment of various ailments.
7-12
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EXTRACT OF 1:5 MIXTURE OF FIVE MOROCCAN MEDICINAL PLANTS HAS CYTOTOXIC EFFECT ON SOME HUMAN CANCER CELL LINES
*Latifa Doudach, Laila Benbacer, Bouchra Meddah, My El Abbes Faouzi, Khalil Hammani, Mohammed El Mzibri and Yahia Cherrah
 Abstract                  View                 Download                 XML
Traditional medicines have long been used by the Moroccan people. Several medicinal recipes prepared from a mixture of plants are often used by traditional medicinal practitioners for the treatment of many diseases including cancer. However, little is known for their scientific information especially their anticancer effects. In this study, one traditional used Moroccan herbal remedy for treatment of hemorrhoids and cancer therapy (cervical cancer, skin cancer) was investigated for its cytotoxic effect against cancer cell lines. Aqueous herbal distillate of mixture in equal quantity of five medicinal plants was evaluated for its in vitro cytotoxicity, using the 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, against two human cancer cell lines; cervix adenocarcinoma (HeLa) and breast cancer cell line (MCF7). The mixture of plants exhibited potent cytotoxicity with an IC50 of 60 &micro;g/ml for Hela cells and 122 &micro;g/ml for MCF7 cells. Furthermore, the mixture was evaluated in vitro for antioxidant potential with the spectrophotometric method based on the reduction of the stable DPPH (1,1-diphenyl-2-picryl hydrazyl) free radical. The IC50 value was calculated in order to evaluate the antioxidant efficiency of plants mixture. The Aqueous herbal distillate of mixture exhibited moderate antioxidant activity 62.76 %, with an IC50 value of 96.8 &micro;g/ml compared to the IC50 value of 4.73 &micro;g/ml as shown by the reference antioxidant Trolox. In conclusion, this herbal remedy that was used to treat cancer patients has effective cytotoxic effect on cancer cells in vitro, and further studies of the active extracts are necessary for chemical characterization of the active compounds and more extensive biological evaluations.
301-307
9
IN VITRO ANTIBACTERIAL ACTIVITY OF ROSMARINUS OFFICINALIS METHANOLIC AND AQUEOUS EXTRACTS
*Rachad Alnamer, Katim Alaoui, Latifa Doudach, El Houcine Bouidida, Fatiha Chibani, Mosa’d AL-Sobarry, Abdelaziz Benjouad and Yahia Cherrah
 Abstract                  View                 Download                 XML
Rosemary (Rosmarinus officinalis L., family Lamiaceae) had been reported in traditional medicine, to exhibit antimicrobial properties. Therefore, this study is aimed at determining the antibacterial activity of R. officinalis leaves against pathogenic microorganisms by determination the minimal inhibitory concentration and to serve as criteria to recommend the ethno pharmacological uses of the plant. Plant leaves were dried, powdered and extracted by cold maceration with methanol for 48h. The extracts were screened against 24h broth culture of bacteria seeded in Muller Hinton Agar at concentration 200, 100, 50, 25, 12.5, 6.25, 3.125 and 1.56mg/ml in sterile distilled water and incubated at 37&deg;C, for 18h and measuring the inhibition zone diameter (IZD). Minimum inhibitory concentrations (MICs) against three Gram-positive bacteria (Micrococcus luteus, Staphylococcus aureus and Bacillus subtilis), three Gram-negative bacteria (Klebsiella pneumoniae, Pseudomonas aeruginosa and Escherichia coli) were determined for the methanolic and aqueous extracts of Rosmarinus officinalis. The methanolic extract showed pronounced antibacterial than the aqueous extract against all of the tested microorganisms. Methanolic extract inhibited with minimal inhibitory concentration of 1.56, 1.56, 3.13, 1.56, 3.13 and 3.13mg/ml against Micrococcus luteus, Staphylococcus aureus, Bacillus subtilis, Klebsiella pneumoniae, Pseudomonas aeruginosa and Escherichia coli, respectively, while the aqueous extract inhibited with minimal inhibitory concentration of 6.25 mg/ml against all tested bacterial strains both Gram-positive and Gram-negative bacteria. The methanolic and aqueous extracts of R. officinalis demonstrated activities against certain bacteria confirming the use of the plant in ethno pharmacology.
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