Volume 5 - Issue 1

S.NO Title & Authors Name page
1
ANTITUMOR EFFECT OF THE INFUSION OR PLUMBAGIN METABOLITE OF PLUMBAGO PULCHELLA BOISS WHEN ADMINISTERED TO CD-1 MICE WITH L5178Y LYMPHOMA CANCER CELLS
Georgina Almaguer Vargas; Miguel A Villavicencio Nieto; Blanca Estela Perez Escandon; Alejandro Chehue Romero; Lauro Figueroa Valverde; Guillermo Manuel Gonzalez Hernandez, Diana Andrea Altamirano Baez, Jose Ramon Montejano Rodriguez
 Abstract                  View                 Download                 XML
Cancer is a group of diseases with a high incidence worldwide. Since existing treatments have extremely strong adverse effects and are often inaccessible to many patients, it is necessary to seek alternatives. The aim of this study was to explore the effectiveness of infusing Plumbago pulchella Boiss and its main metabolite, plumbagin, on L5178Y tumor growth and survival time of the animals. The acute toxicity of Plumbago. pulchella Boiss infusion was also evaluated. The results showed that the antitumor effect both infusion and plumbagin was the same, regardless of the concentration or the route of administration (oral / intraperitoneal), nor which is given as a single exposure or every 48 hours. The tumor size was inhibited between days 8 and 14 of treatment; however there was no increase in survival time of the animals. The Infusion of Plumbago pulchella shows no toxic effects. No macroscopic alterations were noted in the viscera of the treated rats.
1-8
2
DEVELOPMENT OF A SIMPLE HPLC METHOD USING CORE-SHELL PARTICLES COLUMN FOR QUANTIFICATION OF IR3535 IN INSECT REPELLENT LIQUID FORMULATIONS.
Sinh Thai Nguyen, Jean-Paul Salvi, Elodie Gilbert, Celine Serre, Fabrice Pirot, Roselyne Boulieu
 Abstract                  View                 Download                 XML
A simple, fast, precise and sensitive method for the quantification of IR3535, an insect repellent used in topical formulations, was developed by reversed-phase high performance liquid chromatography. The separation was carried out by using a core-shell silica particles column (Kinetex  C18, 5??m, 4.6 mm x 150 mm) with a mobile phase consisting of 0.1% formic acid and methanol (40 : 60 v/v) in an isocratic mode at 0.5 ml/min and UV detection at 220 nm. The calibration curve was linear from 10 to 400 ??M, with regression coefficient r2 = 0.9999. Limit of detection (LOD) and Limit of quantification (LOQ) were found to be 1 and 10 ??M, respectively. Precision and accuracy fulfilled the acceptance criteria. This method was validated and may be routinely used for quantitative analysis of IR3535 from various topical insect repellent liquid formulations.
9-14
3
AWARENESS OF SAFE USE OF OVER-THE-COUNTER DRUGS AMONG NURSING STUDENTS
Suzana Mlinar, Rosanda Raskovic Malnarsic
 Abstract                  View                 Download                 XML
The aim of the present study was to determine the degree of awareness regarding safe use of over-the-counter drugs. The study population included nursing students of the Faculty of Health Sciences in Slovenia. A cross sectional study was conducted between Feb’and Apr’2013. Descriptive statistics were determined to illustrated participant's characteristic. A t-test was used to determined gender-based differences and the contingency coefficient for determining differences per place of residence, age, the consultant for the use of over-the-counter drugs and the number of over-the-counter drugs taken within the last month. The majority of the participants will read instructions for safe use before using the over-the-counter drug, with a statistically significantly higher incidence in women (p = .021). Participants re-read the instructions after deciding to increase the dosage of the over-the-counter drug, with a statistically higher incidence in men (p = .014). It is important to raise awareness of the seriousness of self-medication among nursing students. Thus, safe use of over-the-counter drugs to improve health.
15-22
4
NON CHOLESTEROL LOWERING DOSE OF ATORVASTATIN AMELIORATES DIABETIC VENTRICULAR MYOCYTE DYSFUNCTION IN MICE
Gulgun Ozansoy, Aslı Ceylan-Isık, Fugen Aktan, Ahmet Cumaoglu, Aysel Arıcıoglu, Cimen Karasu, Nuray Arı
 Abstract                  View                 Download                 XML
The aim of this study was to investigate whether atorvastatin ameliorates diabetes-induced cardiomyocyte dysfunction, independently of cholesterol-lowering effect. Streptozotocin-induced diabetic mice were treated with atorvastatin (10 mg/kg, daily, orally) for two weeks. Ventricular cardiomyocytes were isolated and contractile properties including peak shortening (PS), time to PS (TPS), time to 90% relengthening (TR90) and maximal velocity of shortening/relengthening (+/- dL/dt) were analysed using video-based edge detection. Diabetes caused mechanical dysfunction with dampened stress tolerance of myocytes at high stress frequencies, all of which were significantly alleviated by atorvastatin without affecting hyperglycemia and dyslipidemia. In addition, changes in oxidative stress parameters (CAT activity, GSH and MDA levels) were also normalized by atorvastatin. These data indicate that atorvastatin, independently of its lipid-lowering capacity, reduces myocardial oxidative stress resulting in improved myocyte mechanical function in an experimental model of diabetes. Our results supports the concept that restriction of myocardial oxidative stress is a fundamental goal in the treatment of diabetic cardiomyopathy.
23-32
5
DEVELOPMENT AND COMPARATIVE EVALUATION OF THEOPHYLLINE LOADED EXTENDED RELEASE CAPSULE
Mehmet Ali Ege, Tamer Guneri
 Abstract                  View                 Download                 XML
The aim of the present study was to develop a new theophylline hard gelatin capsule drug delivery formulation. The formulation have been prepared to enhance its dissolution which could provide better oral absorption of theophylline. Therefore, the effects of the component nature and their proportion in the release rate were investigated. Extended release capsules of theophylline were prepared by physical mixing using HPMC and cellulose acetate phthalate (CAP) at various drug-polymer ratios. Hydroxy propyl methyl cellulose (HPMC-E50) ratio was changed the release profile of theophyline. The optimum CAP % was selected as 75 % according to in vitro release studies. Flow properties of the F8 physical mixtures were evaluated by calculation of the Carr’s index, angle of repose and Hausner ratio.The results of the study indicate that new extended release hard gelatin capsules can be promising alternative for the other oral formulations of theophylline.
33-38
6
ONE POT SYNTHESIS, ANTITUMOR, ANTIBACTERIAL AND ANTIFUNGAL ACTIVITIES OF SOME SCHIFF BASE HETEROCYCLES
Smaail Radi, Said Tighadouini, Olivier Feron, Olivier Riant, Yahia Nasser Mabkhot, Salim S. Al-Showiman, Taibi Ben Hadda, Mohammed El-Youbi, Redouane Benabbes, Ennouamane Saalaoui
 Abstract                  View                 Download                 XML
A series of Schiff bases of furan and thiophen rings has been synthesized in one step, in good and excellent yields, using acetic acid as catalyst. The synthesized products were evaluated for their anticancer activity toward breast (MDA-MB231) and colorectal (LoVo) human cell lines cancers. The in vitro antibacterial and antifungal potential of the products were also determined using tree bacterial strains (Echerichia coli, Bacillus subtilis, and Micrococcus luteus) and fungal strains (Fusarium oxysporum f.sp albedinis FAO). Significant activity against breast cancer cell lines and Fungal strains was observed.
39-45
7
SAFETY ASSESSMENT OF EFAVIRENZ AFTER A SINGLE–DOSE BIOEQUIVALENCE STUDY: A TREND TO CORRELATE CENTRAL NERVOUS SYSTEM EFFECT AND PLASMA CONCENTRATION
Marta Vazquez; Pietro Fagiolino; Manuel Ibarra; Laura Magallanes
 Abstract                  View                 Download                 XML
The objective of the current study was to examine safety and pharmacokinetic data obtained from a bioequivalence study of two brands of efavirenz carried out in healthy volunteers in order to assess the relationship between concentrations and appearance of adverse events. Drowsiness was reported in almost all the subjects 1 or 2 hours post dosing and generally the onset of this adverse event was 30 or 40 minutes before high efavirenz plasma concentrations, evidencing a lag time between venous plasma concentration and effect. As many studies reported, arterial drug concentration is higher than the respective venous concentration during drug inputs so adverse events experienced during drug input would correlate with arterial drug concentration rather than the respective venous concentration.
46-52
8
A NOVEL FUSED PYRIDOPYRIMIDINE DERIVATIVES: SYNTHESIS AND CHARACTERIZATION
Mahmoud El-Shahat, Eman A. Elhefny, Ahmed A. El-Sayed, Mowafia A.M. Salama
 Abstract                  View                 Download                 XML
Because of its potent and significant pharmacological activities, The 2-amino-4,6-bis(4-chlorophenyl) nicotinonitrile (1) used as a starting material to synthesis of pyridopyrimidine derivatives 3–7. On the other hand, we used the pyridopyrimidine 4 to synthesis of tricycle heterocyclic derivatives; pyridopyrimidotriazine 8–10; pyridotriazolopyrimidine 11–13 and pyridotetrazolopyrimidine 14.
53-58
9
PRESCRIBING PATTERN OF ANTIHYPERTENSIVES AT A TERTIARY HEALTHCARE FACILITY IN NORTH EASTERN NIGERIA
N. Y. Ikunaiye, S. J. Madu, S. I. Yakubu and J. Muazu
 Abstract                  View                 Download                 XML
Appropriate treatment of hypertension using evidence-based guidelines significantly reduces cadiovascular mobidity and mortality. This retrospective survey was aimed at assessing the prescription pattern of antihypertensive medications in order to ascertain the appropriateness and the extent of adherence to the guidelines. Details on demorgraphy, blood pressure, prescription including number of antihypertensives and number of all medications at diagnosis and at last visit as well as documented co-morbidities/compelling indication were captured from the patients folders. The results highlight high prevalence of hypertension in females (65.69%) compared to males (34.31%). At diagnosis, most female patients presented with stage 2 hypertension  while males presented with stage 1 hypertension based on Seventh Report of the Joint National Committee on the Prevention, Detection, Evaluation and Treatment of Hypertension  (JNC 7) classification of hypertension. Although monotherapy with angiotensin converting enzyme inhibitors (ACEI) was considerable at both visits, polytherapy was dorminant. Thus, prescribers in this healthcare facility adhered to drug treatment guidelines for hypertension.
59-64
10
ANALGESIC AND ANTI INFLAMMATORY ACTIVITIES OF METHANOLIC EXTRACT OF FRUIT RIND OF CITRULLUS LANATUS (THUNB)
Md. Abdul Jalil, Nushrat Jahan, Md. Mohaiminul Islam, A.B. Ripon Khalipha, Chand Sultana Khatun, Md. Mofizur Rahman, Md. A.K. Azad
 Abstract                  View                 Download                 XML
Almost all diseases are associated with the inflammation and pain. The Present study was designed to evaluate analgesic and anti inflammatory potentialities of methanolic extract of Cirtrullus lanatus rind which belongs to Cucurbitaceae family. Potent analgesic activity was observed (p<0.05) in extract 400mg/kg by 66.45% inhibition of writhing reflex and mild (50.63% inhibition) activity was observed in 200mg/kg compare to inhibition of 82.91% of standard Diclofenac 10 mg/kg. On the other hand, anti-inflammatory effect was remarkable (p<0.05) with 400mg crude compare with Standard Diclofenac 5 mg/kg body weight. Dunet test represents 46% reduction of paw edema compare to standard of 56.71%. Preliminary phytochemical screening of the plant extract showed the presence of Glycosides, saponins, flavonoids, steroids and protein were present in the rind of Citrullus lanatus one of which has analgesic, anti inflammatory properties. These provide scientific evidence to support the isolation and development of biologically active components as analgesic and anti-inflammatory agents.
65-68
11
NEW PYRIMIDINE AND FUSED PYRIMIDINE DERIVATIVES: SYNTHESIS AND ANTI HEPATITIS A VIRUS (HAV) EVALUATION
Ahmed Ali El-Sayed, Mahmoud El-Shahat, Samira T. Rabie, Eman M. Flefel, Dina N. Abd-Elshafy
 Abstract                  View                 Download                 XML
Some novel fused pyrimidine derivatives 5–14 were prepared starting with compound 4. Also, Ethyl 4-(2,5-dimethoxyphenyl)-6-methyl-2-thioxo-1,2,3,4-tetrahydropyrimidine-5-carboxylate 2 was used for the preparation of some fused pyrimidine analogs 15–19.  All synthesized compounds were evaluated for their antiviral activity against HAV virus by determination of plaque infectivity count assay on HepG2 cells. Compound 7 has high antiviral activity against HAV even higher than Amentadine (positive control) and on studying its mechanism of action it showed combined action between adsorption and replication of HAV.
69-79
12
STABILITY STUDIES OF THE OPTIMIZED ORAL CONTROLLED RELEASE VERAPAMIL HYDROCHLORIDE TABLET FORMULATIONS
Ahmad Khan, Jallat Khan, Hafiz Shoaib Sarwar, Kamran Hidayatullah, Amina Riaz, Zanib Chaudhry
 Abstract                  View                 Download                 XML
In the present study accelerated testing (6 month) and long term testing (12 month) were carried out on Oral Verapamil Hydrochloride control release tablet in order to assess the physical and chemical stability of Verapamil Hydrochloride tablets. All the formulations were tested for disintegration test, % drug content and % drug release over the entire period of testing. These formulations did not show any significant change in any parameter during 12 month and 6 month of testing at 25+5?C/60+5% RH and at 40+2?C/75+5% RH, respectively. All the results were within the acceptable limits. Shelf lives calculated by software R Gui were found to be 43.452, 43.577 and 43.234 months at 1, 3 and 6 months for F4 in accelerated stability. However shelf lives were 44.112, 41.634, 41.867 and 42.896 months at 1, 3, 6 and 12 months respectively in long term stability.
80-86
13
THE INFLUENCE OF PHARMACEUTICAL CARE TO IMPROVE THE KNOWLEDGE AND ATTITUDE CLIENT BY SELF MEDICATION OF NON STEROIDAL ANTI INFLAMMATORY DRUG (STUDIES IN SEVERAL PHARMACIES IN SURABAYA-INDONESIA)
LizaPristianty, Fasich, Arief Wibowo, Mufarrihah
 Abstract                  View                 Download                 XML
Currently self medication NSAIDs getting much done in the community. Efforts are made by using drugs on their own without any guidance from health professionals. The fact that there is a lot done despite such action, but not many people who know choose and rational use of NSAIDs. NSAIDs are widely used in self-medication as an analgesic, antipyretic, and anti-inflammatory. The main side effects are nausea, vomiting, diarrhea, stomach bleeding, and dyspepsia. NSAIDs area class of drugs can be delivered directly to the client by the pharmacist at the pharmacy. A pharmacist as professionals in the pharmacy has a major contribution to the client's decision to self-medication NSAIDs. The purpose of this study was to determine the effect of the practice of pharmaceutical care by pharmacists during a service clients NSAIDs by self medication to increase knowledge and change attitudes of clients, thus expected to achieve an optimal therapeutic effect.This study was an experimental study that conducted 7 training to pharmacists in pharmacy practice in Surabaya with a guide booklet, the pharmacist then implement pharmaceutical care in the service of self-medication NSAIDs to their clients follow the guidelines in the booklet. Monitoring results of interventions by pharmacists in selected pharmacies were evaluated using pre Experimental Design type-One Group Pre Test Post Test Design. The results showed pharmaceutical care by pharmacists in the service of self medication with NSAIDs guide booklet significant effect on attitude change in the use of NSAIDs clients with Sig. (2-tailed) 0.03=0.05andsignificant effect on the improvement of the client's knowledge of the NSAIDs with the Sig. (2-tailed) 0.00 =0.05.
87-92
14
RAPID SINGLE STEP GREEN SYNTHESIS OF COPPER OXIDE NANOPARTICLES FROM VIGNA RADIATA USING THREE COPPER SALTS AND STUDY ITS ANTIMICROBIAL NATURE
Monalisa Chakraborty, Anindita Dey, Niranjan Bala, Sukhen Das, Ruma Basu, Papiya Nandy
 Abstract                  View                 Download                 XML
18-50nm sized copper oxide nanoparticle has been synthesized by an ecofriendly method where seed electrolyte of Vigna radiata act as a phytoreducer to reduce three different copper salts separately [copper (nitrate, sulphate, chloride)]. Fastest reaction followed by smallest nanoparticle was obtained when electrolyte reacted with copper nitrate. Morphology and charge distribution of nanoparticle was confirmed by characterization and to indentify different phytochemicals which are present in electrolyte, different phytochemical assays were performed by standard techniques. This experiment showed that phenols and flavonoids present in the electrolyte are the main phytoreducer to generate negatively charged copper oxide nanoparticle. Also nanoparticles showed antibacterial activity against S.aureus. So the experiment specified that nanoparticle could be generated by a low cost, nontoxic, ecofriendly method which could be further applied in different aspects of pharma industry to ameliorate living standard and health care.
93-97
15
DESIGN AND IN VITRO EVALUATION OF DICLOFENAC SODIUM MATRIX TABLETS
Riyaz Ahmed Khan, Kamal Ahmad Qureshi, Gamal Osman Elhassan
 Abstract                  View                 Download                 XML
Diclofenac Sodium is a Non-steroidal Anti-inflammatory Drug commonly used for the treatment of Rheumatoid arthritis, Osteoarthritis, Ankylosing Spondylitis and Periarticular disorders. In this work 100 mgs of Diclofenac sodium Tablets were prepared by Direct Compression method. Pre & Post compression studies were carried out followed by In vitro release studies.
98-106
16
SIMULTANEOUS DETERMINATION OF ESOMEPRAZOLE MAGENISUM TRIHYDRATE AND NAPROXEN BY COMBINED HPLC-CHEMOMETRIC TECHNIQUES AND RP- HPLC METHOD
Amira H Kamal, Mokhtar M Mabrouk, Hamed M El-Fatatry, Sherin F Hammad
 Abstract                  View                 Download                 XML
New chemometric approaches were applied to high performance liquid chromatography (HPLC) for simultaneous determination of esomeprazole magenisum trihydrate and naproxen in their synthetic mixture. These chemometric approaches were applied to the multiwavelength HPLC peak area ratio obtained by plotting the chromatograms at the five wavelengths using diclofenac sodium as internal standard (IS). The multichromatograms were obtained by using the diode array detector (DAD). A mixture of 20 mM acetate buffer (pH=4.2) and acetonitrile (35:65, v/v) was used as a mobile phase on a 5µm (4.6 x 250mm) Inertsil® C18 Column at a flow rate of 1.5 mL/min to separate and determine the investigated drugs in their synthetic mixture. For comparison purposes, a new validated developed classic HPLC method at 299 nm was used to confirm the results obtained from HPLC-chemometric calibration techniques. There was no significant difference between the methods compared.
107-121
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PHYTOCHEMICAL AND MICROBIOLOGICAL EVALUATION OF DIFFERENT CHEMICAL EXTRACTS OF PAPAYA SEEDS ON CLINICAL ISOLATES OF (FGSH HOSPITAL) ISLAMABAD
Muhammad Haseeb Tariq, Bilal Ghaffar, Tauqeer Ahmed, Abbas Sultan, Muhammad Irfan, Muhammad Junaid Farrukh
 Abstract                  View                 Download                 XML
The current study was aimed to evaluate the antibacterial activity of different concentrations of aqueous and non-aqueous extracts of Carica papaya seeds. Dried and grinded papaya seeds (5g), respectively mixed with 95ml of extraction solvent (water, methanol, acetone and ether) for 24 hours were used for this study. Extraction was done by maceration and Soxhlet method. The extract was then decanted and filtered through a Whatman filter paper.  Antimicrobial activities of the each extract were determined using agar well diffusion method using fresh clinical isolates of Escherichia coli and Staphylococcus aureus and their MIC and zone of inhibition were determined. The results were evaluated statistically using paired sample t-test. Aqueous extract of Carica papaya exhibits greater antimicrobial activities against the clinical isolates of Escherichia coli and Staphylococcus aureus as compared to the non-aqueous plant extracts. MIC of aqueous extract against Escherichia coli was 10-3mg/mL, whereas methanolic extracts shows 10-4mg/mL MIC. Similarly MIC of aqueous extract against Staphylococcus aureus was 10-4mg/mL as compared to MIC of methanolic extract 10-6mg/mL. There was statistically significant difference between the zone of inhibition of aqueous extracts of the plant material against Staphylococcus aureus and Escherichia coli (P < 0.001). Preliminary phytochemical analyses reveal that the extracts contain alkaloids, tannins, saponins and phenols. Carica papaya may be used for the treatment of infection caused by Escherichia coli and Staphylococcus aureus.
122-126
18
LARVICIDAL AND INSECTICIDAL EFFECTS OF DIFFERENT EXTRACTS OF MALLOTUS REPANDUS (WILLD.) MUELL.- ARG. LEAF AND STEM AGAINST CULEX QUINQUEFASCIATUS SAY (DIPTERA: CULICIDAE) AND SITOPHILUS ORYZAE LINN. (COLEOPTERA: CURCULIONIDAE)
Md. Rakib Hasan, Nizam Uddin, Md. Mahadi Hasan, Md. Monir Hossain, Mohammad Mostafa Kamal, Kaniz Fatema, H. M. Lutfor Rahman Mazumder, Kabirul Bashar, Md. Sohel Rana
 Abstract                  View                 Download                 XML
This study was designed to evaluate larvicidal bioassay of different extracts of Mallotus repandus against Culex quinquefasciatus and insecticidal bioassay against Sitophilus oryzae in the laboratory. In larvicidal bioassay, methanol extract of leaf, petroleum ether extract of leaf and methanol extract of stem showed good LC50 values (median lethal time) found in three consecutive days (24, 48 and 72 hours). Methanol extract of leaf showed the highest larvicidal activity in each specific time interval. Moreover, the extract also exhibited dose dependent lethal time effect (LT50). In insecticidal bioassay, among all extracts ethyl acetate extract of stem showed the highest insecticidal activity after 24 and 48 hours while petroleum ether extract of leaf exhibited the highest insecticidal activity in 72 hours. Ethyl acetate extract of stem also showed dose dependent LT50. Highly significant (P < 0.0001) relationship between different hours and LC50 values were found in both bioassays. These findings suggest that the extracts have potential toxicity against both insects.
127-133
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SELF CARE TIPS TO IMPROVE THE QUALITY OF LIFE OF WOMEN FOLLOWING MASTECTOMY WITH AXILLARY LYMPH NODE DISSECTION
Sheeba Wiselin.C, S. Kalavathy
 Abstract                  View                 Download                 XML
An axillary lymph node dissection surgery routinely involves the removal of lymph nodes from the (axilla) armpit. These lymph nodes and associated lymph vessels play an important role in the body’s immune system. Axillary lymph nodes help to drain the arm, upper back and chest on that side of the body. The lymph vessels pick up large proteins, toxin, wastes, bacteria, any cancer cells, and about 10-20% of the fluid that circulates throughout the body. Once this fluid enters the lymph vessel it is known as lymph fluid. he lymph vessels drain this lymph fluid into the axillary lymph nodes. The lymph nodes have macrophage cells that break up the proteins and waste. They also make lymphocytes, which helps to fight infection by destroying bacteria and toxins. The removal or impairment of these lymph nodes through surgery or radiation will be a risk for lymphedema. Education is focused on the prevention of anything that restricts lymph flow.
134-136
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DEVELOPMENT OF LIQUID CHROMATOGRAPHIC AND VISIBLE SPECTROPHOTOMETRIC METHODS FOR THE ESTIMATION OF CLOBAZAM IN TABLET DOSAGE FORMS
K. Uma Maheshwar, Golkonda Ramu , Chintala Rambabu
 Abstract                  View                 Download                 XML
An isocratic RP-HPLC method (Method-A) and two visible spectrophotometric methods (Method B and C) were developed and validated for the determination of Clobazam in bulk and tablet dosage forms. In Method-A, a PEAK chromatographic system equipped with Zodiac C-18 (250mm, 4.6mm and 5µm) column, mobile phase of composition 10:30:60 (v/v/v) of tetra hydro furan (THF), methanol (MeOH) and acetonitrile (ACN), UV detector were adopted for the chromatographic analysis. The output signal was monitored at a detection wavelength at 227 nm and integrated by PEAK Chromatographic Software version 1.06. In spectrophotometric methods, the chromogenic reagents such as Fe (II)-1, 10 phenanthroline (Methods-B) and ferricyanide-Fe (III) (Method-C) reagents were used for color development for the estimation of Clobazam in pure and formulations. The developed methods were validated according ICH guidelines and adopted for the assay of clobazam in the bulk drug and formulations.
137-145
21
PRECLINICAL BLOOD CHEMISTRY SAFETY PROFILE STUDIES OF “BASANTA KUSUMAKAR RAS” AFTER CHRONIC ADMINISTRATION TO MALE SPRAGUE-DAWLEY RATS
Kamrun Nahar, Md. Rakib Hasan, Sumon Kanti Chowdhury, Paritosh Chakma, Gulshanara Begum, Nawfel Abdullah, Md. Moklesur Rahman Sarker and M. S. K. Choudhuri
 Abstract                  View                 Download                 XML
Basanta Kusumakar Ras (BKR), an Ayurvedic preparation is used as a traditional medicine in the treatment of Diabetes mellitus. We were eager to know the effect of chronic administration of BKR on the lipid profile, liver function test and kidney function test. To find out the toxicological characteristic of BKR, it was administered chronically to the male Sprague-Dawley rats at a dose of 400 mg/kg. After 28 days chronic administration of the BKR preparation the following toxicological changes were noted. There was a statistically significant increase in serum total cholesterol (TC) and LDL-C level besides a statistically significant decrease in serum HDL-C level; thus leading to a statistically significant increase of both Cardiac Risk Ratio (TC/HDL-C) and Castelli’s Risk Index (LDL-C/HDL-C). There was a statistically significant increase in both bilirubin and creatinine level. Chronic BKR administration revealed dyslipidemia, possible loss of liver function and kidney function.
146-152
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ECONOMIC BURDEN OF UNSTABLE ANGINA/NON-STEMI USING DIFFERENT HEALTH CARE PARAMETERS IN KARACHI
Shazia Alam, Baqir.S.Naqvi, Iyad Naeem Muhammad
 Abstract                  View                 Download                 XML
Unstable angina (UA)  and  non ST segment elevation myocardial infarction (NSTEMI) is one of common ischemic sign of acute coronary syndrome (ACS), a serious emergency medical condition and majority of patients admitted with chest pain, affecting citizens at younger ages leading to more death and disability in low- and middle-income countries thereby having a greater economic impact. The study aim was to determine the economic burden of unstable angina in hospitalized patients by analyzing the cost of different health care parameters in public and private hospitals of Karachi. A prospective study was conducted among hospitalized UA/NSTEMI patients from September 2013 to February 2014. Data was analysed and results were obtained with the help of statistical software SPSS version 20.Overall 227 patients of UA/NSTEMI admitted in private and public hospitals. Majority of them were males 66.5% while 33.5% were females presented with chief complaint of chest pain 41.4%. UA found to have 58.1% and NSTEMI 41.9%. Total hospitalization cost has been estimated approximately $ 34485. Factors like accommodation cost, laboratory charges, medicines cost and angiography contributed differently to increase the economic burden as $ 9675 and $ 6002 whereas significant financial load found to be from medicines and angiography $ 6550 and $12247respectively. Despite advances in treatment, unstable angina/ non ST elevation myocardial infarction UA/NSTEMI presents an enormous medical, social, and economic burden on individuals of Karachi.
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AN EVALUATION OF PACKAGE INSERTS OF ANTIMICROBIAL AGENTS MARKETED IN INDIA
Patel Suhani V, Desai Chetna K, Patel Prakruti P, Dikshit Ram K
 Abstract                  View                 Download                 XML
PI (n=123) of marketed AMA were collected over 6 months period. Quality of information presented was assessed and scores were given for adherence to Indian i.e. Schedule D of Drugs and Cosmetics Rules, 1945 (DCR) of India (0- 20) and US guidelines (0- 35). Adequate font size was printed in 50% PI. Only 4% PI were complete as per DCR. Adequate information on contra-indications (25%), use during pregnancy/lactation (82%), warning/precautions (92%), drug-drug (59%) and drug-food interactions (36%), adverse drug reactions (61%), effect on ability to drive/use machines (17%), overdosage (36%), uses (48%), pharmacodynamic (32%) and pharmacokinetic properties (61%), use in children (80%) and elderly (52%), preclinical study data (33%), post- marketing surveillance data (7%), references (12%), recent major changes (10%) and patient counseling information (16%) was included in few PI. A more meticulous approach in preparation of PI to include approved, essential, accurate, evidence-based and updated prescribing information is needed.
158-164
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HEPATOPROTECTIVE ACTIVITY OF MUSA SAPIENTUM FRUIT PEEL (MSPE) AGAINST STREPTOZOTOCIN -INDUCED TOXICITY IN RATS
S. Sathiya Narayana Murthy, Christilda Felicia Jebakani, S. Sundara pandian, S. Ponmozhi
 Abstract                  View                 Download                 XML
In this study, to assess the Hepatoprotective activity of Acetone extract of Musa sapientum fruit peel (MSPE) against Streptozotocin (STZ) induced hepatotoxicity in rats. Totally Thirty six male albino wistar rats were divided in to 6 groups; Group I received distilled water; animals in the Groups  II,III,IV,V are induced single injections STZ 45mg/kg intraperitonialy; Groups IV,V,VI received MSPE in doses of 200,400,800 mg/kg/day respectively. The result obtained in this work was determined the MSPE at the doses 200 and 400 mg/kg/day showed significant hepatoprotective effect by reducing the serum marker enzymes such as Serum Alanine aminotransferase (ALT), Aspartate amino transferase (AST) and Alkaline phosphatase(ALP) and its Histopathological studies further confirmed the hepato protective of MSPE when compared with the STZ induced  hepatotoxicity groups. In the conclusions present study findings of Acetone extract of Musa sapientum fruit peel showed significant hepatoprotective activity compared with control groups.
165-169
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A NEW VALIDATED STABILITY INDICATING HPLC METHOD FOR THE ESTIMATION OF RELATED SUBSTANCES OF LYSINE CLONIXINATE AND CYCLOBENZAPRINE HYDROCHLORIDE IN PHARMACEUTICAL DOSAGE FORMS
Morisetti. Nagendra Kumar, V. Shanmukha Kumar. Jagarlapudi, P. Lohita Varma, Raja Sekhara Prasad . Kottapalli
 Abstract                  View                 Download                 XML
A new gradient reverse phase high performance liquid chromatography method has been developed for quantitative determination of related substances of Lysine Clonixinate (LC) and Cyclobenzaprine Hydrochloride (CBP) in pharmaceutical dosage forms. Chromatographic separation achieved on a column L1, C18  250 mm x 4.6 mm; 5 µm with 0.025M Potassium dihydrogen phosphate pH 3.0 buffer as mobile phase-A and Acetonitrile as mobile phase–B at a flow rate of 1.7 mL min-1. Diluent as water and acetonitrile of ratio 50:50 v/v with detection at 245 nm. The injection volume was 20 µL and a gradient program with  run time of 60 minutes. The developed method validated according to ICH guideline with the parameters specificity, forced degradation (with stress conditions of acid, base, oxidative hydrolysis, thermal and photolytic degradation), limit of detection and limit of quantification, linearity, precision, intermediate precision, accuracy, robustness and ruggedness. From all validation parameters results the method proved as specific, stability indicating, precise, accurate, robust and rugged method.
170-179
26
STUDIES ON MEDICATION ADHERENCE AND QUALITY OF LIFE IN TUBERCULOSIS PATIENTS
Nasara Reddy Nakka, M. Geeravani, M. Kranthi kumar, Tejaswi.V, K. Mudiyarasan, G. Gopala krishnan
 Abstract                  View                 Download                 XML
Tuberculosis is a serious public health issue causing immense morbidity, mortality, and distress. This prospective study was conducted at DOTS centre in the Department of TB&CHEST at RMMCH. Pre-determinant questionnaire was used to assess the patient’s knowledge and adherence before and after counselling. Pill Count Method was used to assess the medication adherence rate on the monthly basis. WHO-QoL BREF which has four domains was used to assess the impact of TB on QoL. Before treatment, scores in all domains were significantly lower in patients than controls, more affected were psychological and social relationship domains. Gradual increase in scores was observed over the course of the treatment and counselling. Hence patient counselling shows positive impact on understanding their illness, the role of the medications in the treatment for improving adherence rates. This would enable health care professionals to devise relevant interventions such as patient counselling which would be useful in further improving quality of TB control programs.
180-189
27
FERTILITY EFFECT OF CYCAS CIRCINALIS.L EXTRACT ON MALE WISTER RATS
B. Senthil Kumar , J. Vijaya kumar
 Abstract                  View                 Download                 XML
Infertility is a major public health concern. In Siddha System of Medicine many herbs were used for treating male sexual disorders. The use of herbs remarkable increased over the past few years and researcher now focuses on herbs. The present study was taken to analyze the fertility effect of an herb Cycas circinalis on male albino rats. A total of 18 healthy adult male albino rats were taken and divided into 3 groups with 6 rats in each group. One group of animal was administered orally Cycas circinalis extract (200mg/kg bodyweight) and compared to the normal control and positive control albino rats given testosterone 10µg/kg body weight subcutaneously. Various parameters were compared among the groups and the drug’s efficacy was analyzed. The administration of the drug showed significant positive results in positive control followed by experimental group. Since the synthetic hormonal preparation have grave side effects it’s better to go with herbal aphrodisiacs for better results without any side effects.
190-195
28
STABILITY INDICATING RP-HPLC METHOD FOR THE SIMULTANEOUS DETERMINATION OF OLMESARTAN MEDOXOMIL AND ATORVASTATIN CALCIUM
Shankar Ganesh Gadepalli and Ramakrishna Sistla
 Abstract                  View                 Download                 XML
A simple, rapid, and precise, stability indicating RP-HPLC method for simultaneous analysis of olmesartan medoxomil (OLM) and Atorvastatin (ATR) in bulk has been developed and validated. The analytes were separated by using a Waters symmetry C18 analytical column (250mm × 4.6mm, 5 µm) with a mobile phase consisting of Acetonitrile and 0.02 M potassium dihydrogen phosphate buffer containing 0.1% heptanesulphonic acid sodium (pH 3.0, adjusted with o-phosphoric acid) in the ratio of 55:45 (v/v) at a flow rate of 1.2mL/min. The chromatographic separation was monitored at 253 nm with a run time of 10 min. A volume of 20 µL was injected into the system. Olmesartan medoxomil and Atorvastatin were eluted with approximate retention times of 4.15min and 7.36 min respectively. Calibration plots were linear over the concentration ranges 0.5 to 10µg mL-1 for both the drugs. The high recovery and low coefficients of variation confirms the suitability of the method for simultaneous analysis of the two drugs in formulations also.
196-201
29
SYNTHESIS AND ANTIBACTERIAL ACTIVITY OF PYRIMIDINE DERIVATIVES
Ayushi Uniyal, Alka N. Choudhary, Preeti Kothiyal
 Abstract                  View                 Download                 XML
A series of 3-amino-4,6-diphenyl-3,4-dihydro-1H-pyrimidine-2-thione derivatives  have been synthesized by cyclization of substituted chalcones with thiosemicarbazide. The structures of all newly synthesized compounds were confirmed  by FT- IR, 1H NMR and Mass spectral analysis. The synthesized compounds were screened for  antibacterial activity (500µg/ml) concentration level by disc diffusion method against (Gram negative) Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumonia.
202-206
30
IMPACT OF CLINICAL PHARMACIST ON \"PATIENTS CARE\" IN ACUTE CORONARY SYNDROME
Kota Lakshmi Narasimha rao, G Nikhitha Reddy, N Vinny, Soumadip Das, C K Dhanapal, S Selvamuthukumaran
 Abstract                  View                 Download                 XML
This is a single blinded interventional research in which patient were regularly followed-up. First Intervention includes collecting data to analyze cost of therapy which includes cost minimization by prescribing cheap brands/generics (pharmacoeconomics), quality of life of the patients was measured by WHO QOL BREF questionnaire as outcome of Intervention and second is early ADR detection and prevention and life style modification. The overall mean score of WHO QOL BREF questionnaire of all recruited patients at the time of admission was 30.39 which comparatively increased to 58.45 at the time of discharge. The total cumulative therapy cost was accounted Rs.451, 320.98 for all 65 patients included in the study. The average no. of drugs prescribed per prescription were approximately 7 drugs and 6 drugs in generic name and as per WHO essential list respectively. Patient counseling and life style modifications in addition to treatment showed significant evidence in improvement of patients’ therapeutic outcomes, improved quality of life.
207-212
31
A REVIEW ON PATCH VACCINATION AS A MODERN FORM OF DRUG DELIVERY
Anagha Shivaji Patil
 Abstract                  View                 Download                 XML
Nanopatch is a modern technique which designed to target the network of immunologic antigen presenting cells in the dermis and epidermis layers under the skin to enhance the efficacy of vaccination. Nano patch contains the micro sized needles that are painlessly penetrating and the nano-formulated medicines, drugs and vaccines directly go to the immune system. The comparative studies outline that there is increased stability, conventional route, easy to use and also the wide range of vaccines can be used.  Nano patch one day will be self-administered or given by a non-medical person, which reduces the administration cost and also relieve from burden on health care professionals. Receiving vaccines from doctor’s office or health clinic may soon be outdated.
213-218
32
HESPERIDIN A BIOFLAVONOID MODULATES THE EXPRESSION LEVELS OF SNCA AND PARKIN IN 6-HYDROXYDOPAMINE INDUCED NEUROTOXICITY IN RATS
Priya Nagappan, Vijayalakshmi Krishnamurthy
 Abstract                  View                 Download                 XML
Parkinson disease (PD) is one of the neurodegenerative disease and oxidative stress plays a vital role in its causation. The present study was carried out to evaluate the role of hesperidin in the expression of SNCA, LRRK2, Parkin and PINK1 during 6 hydroxydopamine induced Parkinson rat model. Animals were divided into 5 groups: GroupI served as normal. GroupII was induced with 6-hydroxydopamine (8µg/2µl in 0.1% ascorbic acid-saline). Group III: 6 hydroxydopamine + 50mg/kg b.w hesperidin. GroupIV: 6-hydroxydopamine + 50mg/kg b.w hesperidin+100mg/kg b.w of L-Dopa. GroupV: 6-hydroxydopamine+100mg/kg b.w L-Dopa. The mRNA and protein expression of SNCA, LRRK2, Parkin and PINK1 was evaluated. Hesperidin 50mg/kg b.w and L-Dopa 100mg/kg b.w treated rats showed better results. It may be attributed that treatment with hesperidin and L-Dopa in combination suppress the expression level of SNCA and LRRK2, while it enhanced the expression level of parkin and PINK1. By western blot analysis, group IV treated animals showed suppressed protein levels of SNCA and LRRK2 genes while elevation in the protein level of Parkin and PINKI was noticed. The findings of these studies show that hesperidin can ameliorate 6 hydroxydopamine induced degeneration of dopaminergic neurons during Parkinson disease.
219-224
33
ADVANCES IN VETERINARY DOSAGE FORMS: AN UPDATED NOTION
Om Bagade, Neha Nemlekar, Riddhi Patel
 Abstract                  View                 Download                 XML
Animal provide us with companionship, recreation, and manual labor. Just like a human these  animal receive medicine to keep them healthy, and the reasons for producing single dose veterinary dosage forms is the same as those in humans; to permit delivery of an active in a forms that is effective, safe, and able to  handled and administered by the end user. This review provides the basic background in the design and evaluation of veterinary dosage forms. The basic dosage forms are describe according to their pharmaceutical characteristic, whereas the description of advanced drug delivery system are organized according to route of administration. In contrast in the veterinary field, the major reasons for developing a drug into a long-acting drug delivery system is to minimize animal handling to reduce the stress to the animals from repeated administration and to reduce the cost of treatment in the terms of money and time spent by the end user on drug administration.
225-237
34
IN VITRO ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACTS OF SOLANUM ERIANTHUM D. DON
Radhika Mahadev, Ramakrishnaiah Hanumanthaiah, Krishna Venkatarangaiah, Naveen Kumar Narayanaswamy, Deepalakshmi Arjunan Paranthaman
 Abstract                  View                 Download                 XML
The present study involves assay of the in vitro antioxidant activity of methanolic extracts of leaf, stem, fruit and root of Solanum erianthum. The in vitro antioxidant activity was evaluated by using free radical scavenging studies: DPPH radical scavenging, hydroxyl radical scavenging, hydrogen peroxide, reducing power, phosphomolybdate, total phenol and total flavonoid assay. The methanolic extracts exhibited a dose-dependent scavenging activity of DPPH, hydroxyl radical and hydrogen peroxide. All the extracts showed significantly higher inhibition percentage and positively correlated with total phenolic content. In addition, the total antioxidant assay established the antioxidant property of the methanolic extracts. Fruit and leaf extracts proved to be effective for the said parameters.
238-243
35
EVALUATION OF DIURETIC ACTIVITY OF POLYHERBAL FORMULATION
Vaijayanthimala Palanisamy, Sureshkumar Shanmugam, Sangameswaran Balakrishnan
 Abstract                  View                 Download                 XML
The present study was carried out to investigate the diuretic effect of ethanol extract of the leaves of Trichosanthes cucurmena L. (EETC), Cucumis sativus L.(EECS) and fruits of Corriandrum sativum L. (EECRS)  to make a poly herbal formulation (PHF) and  were administered to experimental rats orally at the dose level of  of 150mg/kg and compared with standard drug  Furosemide (20mg/kg). The diuretic effects of the extracts and PHF were evaluated by measuring the parameters like urine volume, sodium, potassium and chloride contents. The lipschistz method used in rat for the experiment purpose.  The extract and PHF showed a marked level of increase in urine volume and electrolytes like Na+, K+ and  Cl-  ion concentration. The moderate   diuretic effect was observed and significant diuretic effect from PHF . PHF (150mg/kg) showed more diuretic effect than standard. This might be the first formal report on diuretic effects of these three plant combinations.
244-247
36
A REVIEW ON RISKS INVOLVED IN STATIN THERAPY
V. Bhavan Kumar, Avinash A, K. Narendra, I.V.V.S.S. Kartheek Kumar, S. Parimala krishnan
 Abstract                  View                 Download                 XML
There is a controversy going on statins concerning their safety in patients, since their introduction in 1987. Statins known to show a lot of beneficial effects in treatment. However, there are number of risks associated with statin therapy such as elevated blood sugar and enzyme levels, memory impairment, myopathy, rhabdomyolysis and congenital abnormalities. The diabetes risk should be taken into account only if statin therapy is considered for patients at low CV risk, the risk of diabetes mellitus was found to be minor when compared to cardiovascular benefits. Cognitive impairment such as memory loss was found from high dose use of statins and resolves on discontinuation of therapy. In statin therapy muscle toxicity is rare however creatine kinase (CK) levels should be monitored. It is advisable to avoid statins during pregnancy. Finally, overall benefits of statin therapy takes advantage over the risks.
248-252
37
MEASURING SERVICE QUALITY OF PHARMACIES-A STUDY WITH REFERENCE TO THREE MAJOR INDIAN CITIES
P. Uma Maheswari Devi, B. Sankara Rao, B. RajaShekhar, N. Udaya Bhaskar
 Abstract                  View                 Download                 XML
The Pharmacists have a very important role in protecting the health of the customers through proper dispensing of drugs and providing better services. But the errors made in processing prescriptions are growing with the increasing prescriptions. In this scenario we made an attempt to measure the quality of services provided by pharmacists. CFA (Confirmatory Factor analysis) was used and a PHARMQUAL scale with three dimensions-Empathy, Assurance and Reliability and nine items were developed. The relationships between the pharmacists “perceived service quality” and “satisfaction” were also examined. The analysis of data was conducted through Structural Equation Modeling (SEM) using AMOS (Analysis of Moment Structures). A Sample of 350 chemists from three major Indian cities was purposively selected. The role of organized and unorganized pharmacies was also examined. This study is very useful for the doctors, manufacturers (pharmaceutical companies) and customers to evaluate the role of the pharmacist and identify the deficiencies in the quality of pharmacy practice.
253-264
38
MEDICINAL USES AND BIOLOGICAL ACTIVITIES OF SOPHORA INTERRUPTA BEDD-A REVIEW
Pardhasaradhi Mathi, Venkata Raman Bokka and Mahendran Botlagunta
 Abstract                  View                 Download                 XML
Sophora interrupta Bedd, was a common woody perennial herb native to India. Reports suggest that the plant possesses numerous health promoting benefits such as anticancer, antiinflammatory, antimicrobial and antioxidant activities. The plant exhibited several potential in vitro anticancer effects on MCF-7, PC-3, HeLa and HePG-2 cell lines. In vivo assay’s suppressed in mouse models Dalton’s lymphoma ascites, along with acute toxicity studies, central nervous system and hepatoprotective studies. The major phytoconstituents isolated from this plant are prenylated flavonoids, chalcones, biochanin-A and Kaempferol. This review aims to concern about taxonomy, cultivation, biological activities of the isolated compounds and to evaluate the current status of S. interrupta to give a comprehensive view of its future development and further improvement of its suitability in curing a wide range of ailments.
265-273
39
IN VITRO ASSESSMENT OF ANTIARTHRITIC ACTIVITY OF SPHAERANTHUS AMARANTHOIDES BURM F.
Gayatri S, Uma Maheswara Reddy C, Chitra K
 Abstract                  View                 Download                 XML
Present study was done to assess the in vitro antiarthritic activity of the plant Sphaeranthus amaranthoides Burm F (Asteracea). Four different extract of increasing polarity namely petroleum ether, chloroform, ethyl acetate and methanol were prepared. Antiarthritic potential of the plant was assessed by two methods namely, effect on protein denaturating and effect on membrane stabilization. The results indicated that by both the methods ethyl acetate extract was having highest activity and the activity was comparable with that of standard, diclofenac sodium.
274-277
40
DEVELOPMENT AND EVALUATION OF NASAL IN SITU GEL FORMULATIONS OF ALPRAZOLAM USING IN VITRO AND IN VIVO METHODS
Hemanth Kumar Mamidi, Madhavi Harika Srimathkandala, Krishna Sanka, Madhu Babu Ananthula, Vasudha Bakshi
 Abstract                  View                 Download                 XML
The objective of the present research work was to develop and evaluate the nasal in situe gel formulations of alprazolam for better availability in the brain. Formulations were developed using Pluronic F127 and sodium alginate by cold method. Formulations were evaluated for permeation study through sheep nasal mucosa, histopathological evaluation of mucosa and pharmacodynamic study in rats. Optimized formulation showed a diffusion of 78.75 ± 0.077 % drug in 240 min, effective permeation coefficient (Peff) and gelling temperature were found to be 6.44×10-5 cm sec-1 and 33.80 ± 0.57°C respectively. Histopathological study did not show any damage to the nasal mucosa during permeation. The locomotor activity and anti-anxiety effect of Alprazolam differed significantly by I.N and I.V routes compared to control. It can be concluded that Alprazolam given by nasal route is more effective and show quick onset of action when compared to Intravenous administration of equivalent dose.
278-288
41
TREATMENT OF INCURABLE LIVER CANCER (HEPATOMA) BY FOUR TRADITIONAL HERBAL MEDICINES
M. Rafiq, Muhammad Hanif, Akhtar Ahmed, Asim Awan, Salman Habib, Amir Awan, Ajaz Rasool, Miandad Zardari
 Abstract                  View                 Download                 XML
Prognosis of Hepatocellular carcinoma (HCC) is very poor. Chemotherapeutic agents in allopathic medicines are hardly effective. Long time of survival is possible only in cases of full surgical excision or liver transplantation. Use of herbal medicine in the treatment of liver cancer and other cancers has a long tradition. A total of 30 HCC patients (66% were chronic hepatitis patients) treated with four well known herbal aqueous extracts (viz Bergenia ciliata, Nigella sativa, cassia fistula and Picrorhiza scrophulariflora) in which 17 patients were females and 13 males in age group ranges from 42-72 years. Over all six cases were HBV positive, 11 cases were HCV positive and one case with co-infection HBV and HCV viruses. All others were HBV/HCV negative. All individuals were diagnosed as HCC and further monitored on ultrasound, CT scan and alpha fetoprotein; some of them were confirmed additionally with MRI and tissue biopsy. Their liver function tests and serological tests, to determine the status of HBV and HCV were also performed. Out of 30 confirmed HCC patients 10 showed hundred percent improvements displaying no evidence of mass in liver. Ten patients showed remarkable reduction in their size of tumor/mass and are still on medication. Eight patients were having non compliance who had started treatment but either left in the mid of treatment or lack of follow up while the two patients were passed away. All on medication patients showed remarkable improvement and reduction in the size of tumor. However, no adverse effects were observed. To obtain a convincible data it is proposed to treat all the patients approximately for a minimum period of 5 years.
289-297
42
ANTIHYPERLIPIDEMIC ACTIVITY OF CAMELLIA SINENSIS AND MACROTYLOMA UNIFLORUM ON HIGH FAT DIET INDUCED WISTAR ALBINO RATS
Dhanalaxmi Mohan Raj, K. Bhaskar Reddy
 Abstract                  View                 Download                 XML
Hyperlipidemia is a disorder of lipid metabolism manifested by elevation of plasma concentrations of the various lipid and lipoprotein fractions, which is the key risk factor for cardiovascular disorders (CVD). Camellia sinensis and Macrotyloma uniflorum used as antihyperlipidemic drugs as per literature review. The aim of the present study was to evaluate the antihyperlipidaemic activity of polyherbal formulation of methanolic extract (Camellia sinensis and Macrotyloma uniflorum) in High fat diet (HFD) fed rats. Male Wistar albino rats were randomly assigned to five groups: Groups I normal control; Group II HFD control; Group III HFD+ standard drug (Atorvastatin10 mg/kg), Group IV HFD + Polyherbal methanolic extract (200 mg/kg) and Group V HFD + Polyherbal methanolic extract (400 mg/kg). The whole study lasted for 28 days. Administration of HFD caused a significant (p<0.05) rise in the serum total cholesterol (T.C), LDL-cholesterol, VLDL-cholesterol, triglycerides (T.G). Simultaneous administration of polyherbal methanolic extract of Camellia sinensis and Macrotyloma uniflorum significantly (p<0.05) prevented the rise in serum total cholesterol, LDL-cholesterol, VLDL-cholesterol, triglycerides. There was a significant decrease in body weight and increase in HDL-cholestrol was observed in polyherbal extract treated rats. Thus, the results indicate antihyperlipidaemic effect of Polyherbal formulation.
298-304