Volume 7 - Issue 2

S.NO Title & Authors Name page
1
In vitro antioxidant and α-glucosidase inhibitory activities of aqueous extract and ethyl acetate fraction of hallea stipulosa (rubiaceae)
Poualeu Kamani Sylviane Laure, Wansi Sylvie Lea, Chouna Jean Rodolphe, Miaffo David, Nkeng-Efouet Pepin Alango, Kamanyi Albert
 Abstract                  View                 Download                 XML

Antioxidant potential of plants is useful in the management of diabetes mellitus. Aim of this study was to determine the in vitro antioxidant and a-glucosidase inhibitory activities of aqueous extract and ethyl acetate fraction of Hallea stipulosa. In vitro antioxidant assays were used DPPH (1,1-Diphenyl-2-Picrylhydrazyl) scavenging assay, nitric oxide (NO) inhibition assay, ferric reducing antioxidant power (FRAP), determination of total phenol and flavonoid contents. Aqueous extract and ethyl acetate fraction exhibited a scavenging activity by inhibiting DPPH and NO radicals and showed good reducing power capability. They contain a significant amount of phenols and flavonoids. Ethyl acetate fraction showed a considerable a-glucosidase inhibitory activity with an IC50 of 39.19 µg/ml. Results suggest that antioxidant potential and a-glucosidase inhibitory activity of H. stipulosa could be due to phenols and flavonoids and could be a part of the mechanism by which it manages diabetes.

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2
Evaluation of Gastrointestinal Complications in Lebanese Pregnant Females
Sahar Abo Rida, Sara Alzein, Jihan H. Safwan, Faraj K. Saade
 Abstract                  View                 Download                 XML
Purpose: The aim of this study is to evaluate the knowledge, awareness, and treatment of gastrointestinal complications encountered by females during gestation in Lebanon. 
Methods: A community based retrospective multicenter observational study was conducted. Information regarding frequency, severity, and treatment taken for nausea, vomiting, heartburn, and constipation in pregnant females was recorded. 
Results: We retrieved data for 381 females. The incidence of nausea and vomiting was 77.1%, of whom 53.8% had everyday symptoms and 72% had moderate to severe ones. Medical therapy was sought by 15% of the patients who were prescribed mostly pyridoxine (35.1%) and barely ondansetron (1.4%). Heartburn was reported in 70.9%, who were prescribed antacids (25.2%), histamine 2 receptor antagonists (12.1%), and proton pump inhibitors (10.2%). Constipation was encountered by 33.3% of the females and 11.8% received laxatives to lessen their symptoms. 
Conclusion: Our data show that the management is not very well understood and entails a risk in terms of adequacy and safety.
 
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Toxicological evaluation of Guducyadi Louha, an ayurvedic formulation, on biochemical parameters of sprague-dawley rats
Tanmoy Sana, Md. Mamun Sikder, Nayma Abedin, Md. Al Foyjul Islam, Anindya Barua, Md. Abu Adnan Khan, A. A. M. Rakibur Rahman and M. S. K. Choudhuri
 Abstract                  View                 Download                 XML

Guducyadi Louha (GCD) is an ayurvedic medicine which is used in the treatment of gout. It is important to evaluate its toxicity in the animal system. Therefore, this study aimed to evaluate the toxicological effects of this ayurvedic formulation, Guducyadi Louha in male Sprague-Dawley rats. Acute toxicity tests were conducted to determine the LD50 of the drug. In chronic study, animals were administered with GCD at a dose of and 400 mg/kg body weight for a period of 51 days. The effects of GCD on biochemical parameters (total protein content, lipid profiles, hepatic function test, kidney function test, uric acid level) were measured as indices of organ toxicity. The drug (GCD) did not affect serum total protein and globulin level; however, a significant (p<0.05) increase occurred in albumin and albumin/globulin ratio in the treated group. In lipid profile study, GCD significantly (p<0.05) increased total cholesterol (TC) and Non HDL-C level; thus leading to a statistically significant increase (p<0.05) of both Cardiac Risk Ratio (TC/HDL-C) and Atherogenic Coefficient [(TC - HDL-C)/HDL-C)]. The drug did not significantly (p<0.05) alter the levels of bilirubin, creatinine, urea, urea/creatinine ratio and uric acid. Overall, the alterations in the biochemical parameters especially lipid profile have consequential effects on the normal functioning of the organs of the animals. Therefore, the drug GCD at the dose of 400 mg/kg body weight may not be completely safe as an oral remedy and should be taken with caution if absolutely necessary.

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4
Assessment of errors and incompleteness of prescriptions in outpatient clinics of public sector hospital in Karachi, Pakistan
Farah Khalid, Syed Muhammad Farid Hasan, Fouzia Hassan, Iyad Naeem Muhammad, Sabahat Jabeen, Madiha Mustaque, Fouzia Israr Ahmed, Adnan Iqbal and Azfar Athar Ishaqui
 Abstract                  View                 Download                 XML
Prescribing errors are considered as one of the important source of medication errors which can lead to adverse drug reactions. It can be preventable by limiting the errors in the medications prescribed. In the present study, physician prescriptions of outpatient clinics were evaluated for the completeness of information needed in prescriptions. Around two thousand seven hundred prescriptions were evaluated. An average of five drugs per prescription was prescribed. Medical record number, patient’s height and allergy status of patient was not declared in any of the prescription. Patient’s name (96%), physician contact number (63%), dosage form (83%), strength of drug (71%) and duration of therapy (78%) were mentioned in comparatively good number. A very low number of drugs (10%) were prescribed by generic name. Major drug-drug interactions were found in 18% prescriptions. Omission of essential information from the prescriptions will definitely be the source of medication errors that can be prevented by utilizing services of pharmacists in community pharmacies. 
 
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5
Toxicological studies of an ayurvedic medicine Yogendra Ras used in epilepsy
Mustari Hossain, Md. Rakib Hasan, Majana Khalil, Maksud Hasan Binoy, Md. Mamun Sikder, Tahmina Akter Sony, Afaz Uddin, M. S. K. Choudhuri
 Abstract                  View                 Download                 XML
Yogendra Ras (YR) is an ayurvedic preparation used as a traditional medicine in the treatment of epilepsy. To find out the toxicological characteristic of YR, it was administered chronically to the Sprague-Dawley rats at a dose of 40 mg/kg for 28 days. After 28 days chronic administration of the YR preparation, the following toxicological changes were noted. All throughout the experimental period, the YR treated animals were always maintaining negligible changes in overall body weight. There is a statistically very highly significant (p=0.001; 25.50% decrease) decrease in the absolute weight of the male rat liver and a highly significant (p=0.006; 23.53% decrease) decrease in the relative percent weight of the male rat liver. There is also a statistically highly significant (p=0.002; 18.90% decrease) decrease in the absolute weight of the male rat kidney and a statistically significant (p=0.015; 16.69% decrease) decrease in the relative percent weight of the kidney. There is a statistically significant decrease in the absolute weight of the male rat spleen (p=0.017; 31.10% decrease) and the relative percent weight of the male rat spleen (p=0.021; 30.48 % decrease).
 
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6
An assessment and evaluation of patient compliance in type ii diabetes mellitus in general medicine department of tertiary care hospital
L. Panayappan, Faseela PS, Lincy George, K. Krishnakumar
 Abstract                  View                 Download                 XML

Type 2 Diabetes mellitus or insulin dependent diabetes comprises 90% of the people with diabetic and is a challenging disease to manage successfully. The prevalence is very high in Indians. According to World Health Organization patient compliance towards long term therapy for chronic disease indicates only around 50% in India. A prospective observational survey was carried out for the duration of 8 months among the patients. Medication adherence to diabetes medicine was determined using a modified version of the eight items self-reported Morisky medication Adherence Scale (MMAS).Each item is in a yes/no format, with a maximum possible score of eight equating very poor adherence and zero considered as good adherence. 108 patients were included in the study.54 in the control group and 54 in the intervention group. Intervention group showed more statistical significance (p value< 0.05) for patient compliance as compared with control group. Pharmacist care will significantly improve the therapeutic goal in the achievement of better patient compliance.

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Tolnaftate loaded sln gel for improved transdermal drug delivery: formulation and evaluation
V.Viswanath, B. Narasimha Rao, K. Gnana prakash, D. Sai Padmini, B. Gowthami
 Abstract                  View                 Download                 XML

Solid lipid nanoparticles (SLN) were novel colloidal drug carrier systems offering controlled release profiles for many pharmaceutically active agents. The objective of present research work was to develop Tolnaftate-solid lipid nanoparticles using solid lipid as drug carrier. As the drug was inactive orally, it was delivered by topical route. Hot homogenization along with ultrasonication were used for such purpose. Then they were converted to gel. The drug compatibility was checked by using FTIR studies. Seven different formulations were prepared by variable concentrations of lipids and its effect on drug loading was studied. Produced Tolnaftate- SLN gels  were evaluated for the  parameters like Homogenity, Spreadability, Viscosity, pH, Drug content, Entrapment Efficiency, invitro drug release & invitro drug  permeation studies. Antifungal activity of the optimized formulation (F7) was also studied. Tolnaftate -SLNs have good potential in treating topical fungal infections.

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8
Antianaphylactic Activity Polyherbal Formulation on Mast Cells of Rats against Active Anaphylaxis
C.Girish, Y. Narsimha Reddy
 Abstract                  View                 Download                 XML

Anaphylaxis is an acute systemic reaction, which is produced by the mast cells due to release of different chemical mediators. Anaphylaxis is the result of alteration in physiology of mast cell and is responsible for the various physiological changes. Objective of the present study was to evaluate the antianaphylactic activity of polyherbal formulation against the active anaphylaxis with the help of mesenteric mast cells of rats. The study was carried out by sheep serum induced active anaphylaxis model, triple antigen induced symptomatic active anaphylaxis model and compound 48/80 induced rat paw edema model. The rats which are pretreated with standard drug prednisolone (10 mg) and polyherbal formulation (250 mg) shows beneficial effect against active anaphylaxis. Antianaphylactic activity of polyherbal formulation against active anaphylaxis may be possibly due to the membrane stabilizing potential.

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Study on polypharmacy and potential drug – drug interactions in drug therapy of elderly patients at a tertiary care teaching hospital
Binay Gupta, Jitty Jose, Arun Roy, Divya Jyothi, Rajeswari Ramasamy, TV Venkatadri, Shashidhar G
 Abstract                  View                 Download                 XML

The administration of many drugs at the same time or the administration of an excessive number of drugs can cause the potentially serious drug-drug interactions and adverse drug events. Adverse drug events and serious harms occurs at all ages, although they are more commoner in older people, who are more vulnerable to drug toxicity because of age related physiologic changes and increased risk of disease associated with aging and because of polypharmacy. The objective of this study was to identify the degree of polypharmacy and the frequency of potential drug- drug interactions in the medication regimen of elderly patients. This is a cross – sectional observational study conducted in the general medicine department of a tertiary care teaching hospital over a period of 6 months. Sample size of 150 cases of elderly patients was collected from general medicine ward and was reviewed for polypharmacy and potential drug-drug interactions. From among the 150 cases collected, 65(43%) cases were identified with polypharmacy out of which 23(35%) were male and 42(65%) were female. The average number of drug prescribed per patient was 6.05. Among 150 cases, 71(47%) cases were identified with 152 potential drug-drug interactions out of which 8 cases (11%) were with clinically observed adverse drug reaction due to drug interactions. Majority of the potential drug-drug interactions were pharmacodynamic in nature (67%). It was observed that out of 152 pDDIs identified,17(11%) were major, 110(72%) were moderate and 25(16%) were minor in severity.

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10
Structure Based Drug Design and Synthesis of Ibuprofen analogs as Cox Inhibitors
T.Umema Naaz, Shaheen Begum, Nameera Jabeen, Yerragunta Vishwanadham
 Abstract                  View                 Download                 XML

Cyclooxygenase-2 (COX-2) is one of the important targets for treatment of inflammation related diseases. Structure based drug design was carried out for about 1000 molecules and then molecules having good interactions with 3NT1 protein were synthesized. According to the results of molecular docking, the synthesized novel ibuprofen schiff bases derivatives have selective COX-2 inhibition. In conclusion, the newly synthesized ibuprofen derivatives can be used in model in vivo studies.

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11
Nanosuspensions: A Review
Kamala Kumari, P.V and Srinivasa Rao, Y.
 Abstract                  View                 Download                 XML

The current article cites the importance of the emerging and promising future of new age dosage form, ‘nano suspensions’. Particle size reduction, particularly nanonization, is a non-specific, universal approach to improve the bioavailability of poorly soluble drugs. The article emphasizes importance in the preparation, evaluation and the research work going on with various drugs and their appropriate applications. Nano suspensions have emerged as a promising strategy for the efficient delivery of hydrophobic drugs because of their versatile features and unique advantages. Techniques such as media milling and  high-pressure homogenization have been used commercially for producing nano suspensions. The unique features of nano suspensions have enabled their use in various dosage forms, including specialized delivery systems such as mucoadhesive hydrogels. Rapid strides have been made in the delivery of nano suspensions by parenteral, peroral, ocular and pulmonary routes. Currently, efforts are being directed to extending their applications in site-specific drug delivery.

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12
Antioxidants in Endodontics
Dr. Reena Eknath Dudhe, Dr. N. Vimala, Dr. Leena Padhye
 Abstract                  View                 Download                 XML

In recent years, free radical chemistry has received a great deal of attention. Our body generates Free radicals as reactive oxygen species (ROS) and reactive nitrogen species (RNS) by various endogenous systems and exposure to various physiochemical conditions or pathological states. Oxidative stress results if free radicals overwhelm the body’s ability to regulate them. Free radical thus decreases lipids, proteins, and DNA and leads to number of human diseases. Hence use of external source of antioxidants can help to overcome this oxidative stress. Over the past years, the free radicals and antioxidants have gained tremendous importance in the era of dentistry. These free radicals in the body, can be beneficial or hazardous. Antioxidants are the substances that coincide with and stabilize free radicals thereby guarding and sheltering cells from the harm caused by free radicals. The present article represents a review how free radicals are formed, their interaction in disease pathogenesis, and their potential use in endodontics.

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Development and characterization of Poly (lactide-co-glycolide) microspheres loaded with Flutamide, an anticancer drug for controlled drug delivery
P. Kumara Babu, Y. Maruthi, A. Parandhama, C. Madhavi, H. Sudhakar, U. Sajankumarji Rao, K.V. Sekharnath, M.C.S.Subha and K. Chowdoji Rao
 Abstract                  View                 Download                 XML

Poly (Lactide-co-glycolide) (PLGA) microspheres were prepared through Oil in Water (O/W) emulsion-solvent evaporation method using dichloromethane as solvent. Flutamide (FLT), an anti Cancer drug, was used for encapsulation within PLGA microspheres. Morphology, size, encapsulation efficiency and drug release from these microspheres were evaluated. The Differential scanning calorimetry (DSC) confirmed the molecular level dispersion of Flutamide in the microspheres. Scanning electron microscopy (SEM) studies confirmed the spherical nature and smooth surface of the microspheres produced. X-ray diffraction studies (X-RD) was performed to understand the crystalline nature of drug after encapsulation into the microspheres.  In-vitro release studies indicated a dependence of release rate on the concentration of polymer, the amount of drug loading, but slow release rates was extended up to 14 h.

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A study of awareness about prescription writing among undergraduate medical students: A cross-sectional study
Dr. Mayur M. Sisodiya, Dr. Anuradha Gandhi, Dr Prakruti Patel, Dr Mira Desai
 Abstract                  View                 Download                 XML

Aims: To evaluate the knowledge, attitude and skill of undergraduate medical students about prescription writing. Materials and Methods: The observational, questionnaire based, cross sectional study was carried out in II (group A), III part 1 (group B) and III part 2 (group C) MBBS students. Data was collected through structured, prevalidated questionnaire which included information about demographic details, knowledge about prescription writing, rules and regulation of prescription writing with example to be written by students. Result: A total 475 medical students were divided in group A (n=213), group B (n=144) and group C (n=118). All students knew that the prescription can be written by registered medical practitioner, but only 20.2% students knew that an intern doctor can also write a prescription. Only 40.4% students knew that diagnosis of disease is required in prescription. According to 97.68% students, date of prescription is required to prevent the misuse of blank prescription. Signature of doctor, registration number and contact number of doctor are also important was known by 30.52% students. The refilling information was known by 83.1% students. Prescription was correctly written by 76.05% students of group A followed by group B (56.25%) and group C (34.74%). Total 98.9% students believe that non-pharmacological advice is included in prescription, though 18.52% students did not write non-pharmacological advice in prescription. Conclusion: Students lack detailed knowledge about rules and regulation of prescription writing. Hence a Regular training and evaluation of prescription writing skill can help to promote rational prescribing of medicine.

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Free radical scavenging and antioxidant efficacy of ethanolic fractions from an edible medicinal mushroom - Auricularia polytricha
B. Packialakshmi, G. Sudha, M. Charumathy
 Abstract                  View                 Download                 XML

The main aim of this research was to analyse the free radical scavenging potency and antioxidant capacity of ethanolic fractions from Auricularia polytricha (APEF) fruiting bodies. The free radical scavenging property was evaluated using DPPH (1,1-diphenyl-2-picryl-hydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonicacid)) radical scavenging assay, reducing power, phenanthroline and lipid peroxidation (LPO) inhibition assays and the activity was compared with standard antioxidants. The EC50 values were observed to be 3.15 mg/ml, 3.90 mg/ml, 2.07 mg/ml, 3.12 mg/ml and 6.32 mg/ml respectively. The content of total phenol and flavonoid in APEF were 17.60 mg/g as Ferulic Acid Equivalents (FAE) and 4.96 mg/g as Catechin Equivalents (CAE) respectively. The present study reveals the potential of APEF to scavenge free radicals and the activity was found to be higher in all used in vitro methods. Thus, Auricularia polytricha mushrooms could be used as a potent therapeutic agent and a food supplement.

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Determination of Oseltamivir Phosphate in Capsules by Visible Spectrophotometry
Prasad B, T. Meeravali, N. Durga Bhavani, Y. Rajesh, Sunkara Bhawani
 Abstract                  View                 Download                 XML

A simple, sensitive, accurate and precise spectrophotometric method was described for the determination of Oseltamivir Phosphate in bulk dosage forms. The method is based on the formation of ion-pair of the drug with anionic dye such as bromophenol blue (BPB) which is extracted into chloroform having absorption maxima at 420 nm Regression analysis of the Beer's plots showed good correlation in the concentration ranges 10-100 µg/ml. The proposed method was successfully applied to the dosage forms containing the Oseltamivir Phosphate.   No interference from common excipients was observed.

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GC/MS ANALYSIS, ANTISALMONELLAL POTENTIAL OF METHANOL LEAF EXTRACTS OF TRISTEMMA MAURITIANUM AND EFFECTS ON HEMATOLOGICAL PARAMETERS ON WISTAR RATS INFECTED WITH SALMONELLA TYPHI
David NGOUDJOU TSAFACK, Muhammad ARFAT YAMEEN, Guy Sedar Singor NJATENG, Charles FOKUNANG, Jean Noel NYEMB, Fatima NIGHAT, Donatien GATSING
 Abstract                  View                 Download                 XML

Typhoid fever remains a marked problem of public health in the world especially Africa and Asia. This work aimed to evaluate the antisalmonellal potential of extracts and fractions from Tristemmamauritianum. The microdilution technique was used for MIC and MBC determination whereas GC/MS was used to identify some components of the methanol extract. This extract was tested in vivoagainst salmonella-induced typhoid in rats. Among crude extracts, the methanol extract showed the better MICs ranging from 96 to 192 µg/ml. In vivo, the activity of the methanol extract was comparable to the one of oxytetracycline since both at the tested doses were able to heal infected rats. The hematological parameters showed an increase of WBC and the decrease of RBC and differentials in non treated groups’. From GC/MS 11 compounds were identified using NIST library. The results of this work provide the baseline information for the possible usage of Tristemmamauritianum to treat typhoid fever and others salmonellosis.

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REGISTRATION PROCESS OF GENERIC DRUGS IN USFDA
Mamatha M, Kranthi Kumar B, Ch. Ramya Sree, Teja D, Sravani M, Elphine Prabhahar A, Rama Rao N
 Abstract                  View                 Download                 XML

Regulatory involvement in the generic drug development hastens the drug approval process which directly or indirectly accelerated the launching of drug into the market. The regulatory documents whether in-house of documents to be submitted to regulatory authorities should be carefully reviewed by the skilled personal to minimize the queries raised by the regulatory agencies and speed up the approval process . Sponsors must ensure submissions meet the USFDA requirements for format and content.

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Development and Validation of UV/Visible Spectrophotometric Method for the Estimation of Simavastatin in Bulk and Pharmaceutical Formulations
R. Sai Bharath, A. Aswini, J. Arshiya, J. Nikhil Yadav, G. Siddartha,Paul Richards M
 Abstract                  View                 Download                 XML
Simavastatin is an antihyperlipedemic drug used in the treatment of atherosclerosis a cardiovascular disorder A simple, sensitive, accurate and reproducible UV/visible spectrophotometric method was developed for the determination of Simavastatin in bulk and pharmaceutical dosage forms. The solvent used was distilled water and wavelength corresponding to maximum absorbance for the drug was found at 238 nm. Drug obeyed beer’s law in the concentration range of 10 – 60µg/ml. with a correlation coefficient of 0.999. The linear regression equation obtained was y=0.0565x+0.0249, where y is the absorbance and x is the concentration of the pure drug solution. The method was validated for several parameters such as Linearity, Accuracy, Precision and Robustness as per the ICH guidelines. The % recovery value which is close to 100% indicates reproducibility of the method and absence of interference of the excepients present in the formulation. The authors conclude that the proposed spectrophotometric method for the estimation of Simavastatin can be used for routine analysis of Simavastatin in bulk as well as in tablet dosage form.
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Molecular Docking of Macarangin isolated from Macaranga denticulata with two targets related to diabetes
Abul Hasanat, Arkajyoti Paul, Tanvir Nehal Hossain, Md. Shamsuzzaman, Md. Rahimul Hasan, Mohammed Sohel Meah, Arafat faraque, H.M. Mehedi Hasan, Md. Sajjad Ul Hoque, Mohammad Shah Hafez Kabir
 Abstract                  View                 Download                 XML

To investigation Macarangin, an isolated compound of Macaranga denticulata, can be considered for developing into a potent antidiabetic drug by docking analysis. The plant has used for carminative, emetic, haemoptysis and healing wounds by the tribal in Khagrachari, Bangladesh. Macarangin isolated from Macaranga tanarius was taken as ligand for molecular docking. The molecular targets Alpha amylase and Glucokinase whose crystallographic structures are available on the PDB database as 1PPI, 1V4S respectively, were used for the docking analysis using the Schrödinger-Maestro v10.1. The docking studies of the ligand Macarangin with three different target proteins showed -5.238 and -4.534 respectively Alpha amylase and Glucokinase. According, to the molecular docking it is proved that Macarangin is a potent antidiabetic compound. So, it is need to in vivo investigation in future to identify appropriate antidiabetic activity of Macarangin.

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Antinociceptive activity of methanol extract of Begonia thomsonii A. DC. leaves
Mohammed Farhad, Mohammed Sohel Meah, Ovil Das, Md. Salauddin, Md. Abu Hanif, Md. Rahimul Hasan, Md. Shamsuzzaman, Asifur Rahman, Mofiza Akter, Md. Mominur Rahman, Mohammad Shah Hafez Kabir
 Abstract                  View                 Download                 XML

The nature has provided abundant plant wealth for all the living creatures, which possess medicinal virtues. Therefore, there is a necessity to explore their uses and to ascertain their therapeutic properties. The present study was to investigate anti-nociceptive action of the methanol extract of Begonia thomsonii A.DC. leaves. The mice were submitted to acetic acid-induced writhing test and Formalin induced licking test to assess antinociceptive activities, respectively. Two doses 400 and 200 mg/kg were administered for testing. The methanol extract of B. thomsonii at both doses, exhibited a significant (P < 0.001) dose-dependent antinociceptive activity in acetic acid writhing test and Formalin test. In acetic acid-induced writhing test, oral administration of methanol extract of B. thomsonii (200 and 400 mg/kg) also decreased the writhing significantly while compared to control. The dose 400 mg/kg showed maximum percentage of pain inhibition (33.33%). Aspirin (10 mg/kg) was used as reference antinociceptive drugs. Methanol extract of B. thomsonii (200 and 400 mg/kg) significantly suppressed the licking activity in both phase of the formalin-induced pain in mice in a dose dependant manner.  The  reference  analgesic  drug Aspirin (10  mg/kg)  also  significantly  inhibited the  licking  activity  against  both  phases  of  formalin induced pain. The leaf extract has potential antinociceptive activity. The present study supports the use of B. thomsonii in different pain states.

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MICROWAVE ASSISTED SYNTHESIS, CHARACTERIZATION AND ANTHELMINTIC ACTIVITY OF NOVEL BENZIMIDAZOLE DERIVATIVES
Maneshwar.T, K.Radhika, Rajeswari G.L, Geethanjali.K.B, Umarani.R, Srividya Girija.P.V, Spandana.D.L, Priyanka.S
 Abstract                  View                 Download                 XML
A new class of potentially biological active novel bezimidazole derivatives containing Benzimidazole moiety has been synthesized and evaluation of novel Benzimidazole derivatives for Anthelmintic activity. The title compounds were synthesized in a good yield. The synthesized Compounds 3a-3h was characterized by FT-IR, Mass and 1H NMR data and evaluated for their anthelmintic activities by standard protocol available in literature. All the compounds were subjected for Anthelmintic screening, among this series of compounds 3d and 3e showed high activity against.
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Development and Validation of Stability Indicating RP-HPLC method for simultaneous estimation of Epalrestat and Pregabalin in bulk and tablet dosage form
B. Madhu Harika, Y. Rajendra Prasad
 Abstract                  View                 Download                 XML
The proposed study, a new stability- indicating RP-HPLC method has been developed for estimation of Epalrestat and Pregabalin in bulk and tablet dosage form. The present method was a sensitive, precise, and accurate RP-HPLC method for the analysis of Epalrestat and Pregabalin. To optimize the mobile phase, various combinations of buffer and organic solvents were used on Xterra-(150x4.6mm, 5µ) column. Then the mobile phase containing a mixture of Ammonium acetate buffer (pH 10): ACN 70:30 % v/v was selected at a flow rate of 1.0 ml/min for developing the method and the peaks with good shape and resolution was found resulting in short retention time, baseline stability and minimum noise. The retention times of Epalrestat and Pregabalin were found to be 2.516 min and 3.132 min respectively. Quantitative linearity was obeyed in the concentration range of 37.5-225 and 18.75-112.5 µg/mL of Epalrestat and Pregabalin respectively. The limit of detection and limit of quantification were found to be 0.19µg/mL and 0.57µg/mL (Epalrestat); 0.50µg/ mL and 1.51 µg/mL (Pregabalin) respectively, which indicates the sensitivity of the method. The high percentage recovery indicates that the proposed method is highly accurate. No interfering peaks were found in the chromatogram indicating that excipients used in injection formulations didn’t interfere with the estimation of the drugs by the proposed HPLC method.
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