*Bhawani S, Nagalakshmi Ch and Rohini P Chiral separation in forensic science is chemical separations of optically active isomers of drugs and metabolites. Specific functions of D-amino acids in humans are bound to lead to the revelation of D-amino acid abnormalities in human disorders. Therefore, high-throughput analysis techniques are warranted to determine D-amino acids in biological fluids. Two chromatographic techniques, a nonchiral derivatization with chiral (chirasil-L-val column) separation in a GC-MS system and a chiral derivatization with Marfey’s reagent and LC- MS analysis were developed. The techniques for D-serine, L-serine, and glycine determination in cerebrospinal fluid (CSF) were validated. Toluene monooxygenases (TMOs) have been shown previously to catalyze region selective hydroxylation of substituted benzenes and phenols. TMOs are also capable of performing enantio selective oxidation reactions of aromatic sulfides. <br /> International Journal Of Pharmacy 2249-1848 78 2012-04-01 2 386-391 Copyright (c) Pharma Scholars. All rights reserved 2013