Mahesh H. Kolhe; Ujwala K. Waghmare; Ramesh D. Bhusal ; Pranit P. Hajare ; Swapnil R Chavan A simple, accurate and reproducible RP-HPLC method has been developed for simultaneous estimation of Aspirin and Ticlopidine hydrochloride in tablet dosage form. The RP-HPLC analysis is carried out using Acetonitrile: Ammonium acetate buffer (0.05 M) in the ratio of (68: 32 % v/v) as the mobile phase and MOS Thermosil C8 column (250 mm x 4.6 mm i.d.), flow rate 1.0 mL/min, with detection wavelength of 240 nm. Linearity was obtained in the concentration range of 10-50 mg/mL and 20-100 mg/mL for Aspirin and Ticlopidine hydrochloride respectively. The RP-HPLC methods was developed and statistically validated as per ICH guidelines. International Journal Of Pharmacy 2249-1848 661 2015-10-02 4 1178-1187 Copyright (c) Pharma Scholars. All rights reserved 2013