Syed Aun Muhammad, Humaira Nadeem, Ahmad Sadiq Typhoid fever, caused by Salmonella typhi, is characterized by gastrointestinal illness and fatal bloody diarrhea. The emerging epidemic cases and resistance of this bacterium to broad spectrum antibiotics demands the development of new anti-typhoid drugs. In this study, oxadiazole has been synthesized with significant anti-Salmonella activity. This compound was structurally characterized through FTIR and NMR. The computational docking studies of oxadiazole with essential target protein (UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase) of Salmonella typhi showed stable binding affinity with minimum binding energy of -9.9 Kcal/mol. This computational study will add to our understanding of the drug designing and development, and to target these pathogenic bacteria to treat typhoid infections. International Journal Of Pharmacy 2249-1848 318 2014-01-23 1 252-255 Copyright (c) Pharma Scholars. All rights reserved 2013