Sadia Sultana, Md. Rakib Hasan, Paritosh Chakma, Mohammad Jashim Uddin, Ishtiaque Ahmed Chowdhury, Marjana Khalil, Mohammed Motaher Hossain Chowdhury and M. S. K. Choudhuri Balarista (BLR) is an Ayurvedic preparation used as a traditional medicine to treat debility in the rural population of Bangladesh. To find out the effect of chronic administration of BLR on serum blood chemistry profile, it was administered chronically to the male Sprague-Dawley rats at a dose of 40 ml/kg for 28 days. In this study, the total protein (TP) content was increased (16.15 %) in BLR treated male rats and it was statistically significant (p=0.022). The globulin content was highly significantly (p=0.003) increased (62.83 %) and as a result the decrease (45.16%) in the Albumin / Globulin (A/G) ratio was noticed and it was also statistically highly significantly (p=0.004) different from their corresponding control. There were also a statistically significant decrease of blood urea nitrogen (BUN) level (22.69% decrease; p=0.039) and BUN/Creatinine ratio (26.96% decrease; p=0.021). International Journal Of Pharmacy 2249-1848 608 2015-07-02 3 779-783 Copyright (c) Pharma Scholars. All rights reserved 2013