A VALIDATED RP-HPLC METHOD FOR ESTIMATION OF VENLAFAXINE FROM TABLETS

Chatanyaprasad MK, Vidyasagar G, Sambasiva Rao KRS, Ramanjeneyulu S

An accurate, precise and simple rapid reversed phase high performance liquid chromatographic method has been developed and validated for estimation of venlafaxine in tablet dosage forms. The mobile phase consisted of buffer (pH 3.9; adjusted with ortho phosphoric acid) and acetonitrile at a ratio of 35:65 v/v and mobile phase pumped at a flow rate of 0.6 mL/min with PDA detection at 227nm. The linearity range was found to be 20-60 μg/mL. The method was successfully validated and it was concluded that the developed method was accurate, sensitive, precise, robust and useful for the routine quality control of venlafaxine in pharmaceutical dosage forms.