CHIRAL AGP FOR THE RESOLUTION OF ENANTIOMERS - AN OVERVIEW

T. Sirisha*, B.M. Gurupadayya

Chiral seperation also called chiral resolution,is a procedure used to seperate the two isomers of a racemic compound. Many Chiral stationary phases(CSP’S) have been manufactured for the seperation of enantiomers.Among them Chiral-AGP is the protein based chiral column . α1-acid glycoprotein (AGP) is a very stable protein, which tolerates pure organic solvents,high temperatures and high and low pH. The CHIRAL-AGP is a reversed phase column giving many possibilities to affect both the retention and the enantioselectivity. The solutes are retained by three types of forces Ionic binding (charged solutes), Hydrophobic interaction and Hydrogen bonding. Based on the product type, particle size, internal diameter and column length many columns have been manufactured by Chiral Technologies. Chiral AGP has a broad range of applicability and it has been used for the separation of many basic drugs, environmental pollutents,1,4 benzodiazepines. It is also suitable with MS detection of many drugs.