DEVELOPMENT AND VALIDATION OF A RP-HPLC METHOD FOR ESTIMATION OF HYDROCHLOROTHIAZIDE AND CANDESARTAN CILEXETIL IN PHARMACEUTICAL DOSAGE FORM

*Ananda Rao Bonthala and T.A.D. Surya Kumar

A simple, specific and accurate reverse phase high performance liquid chromatographic method was developed for the simultaneous determination of hydrochlorothiazide and candesartan cilexetil in pharmaceutical dosage form. The column used was Zorbax C8 (150×4.6 mm, 3.5μm) in isocratic mode, with mobile phase containing phosphate buffer-methanol (30:70) adjusted to pH 3.0 using ortho phosphoric acid was used. The flow rate was 1.0 mL/ min and effluents were monitored at 230 nm. The retention times of hydrochlorothiazide and candesartan cilexetil were 2.170 min and 7.280 min, respectively. The linearity for Hydrochlorothiazide and Candesartan cilexetil were in the range of 25-125 mg/mL and 16-80 mg/mL respectively. The recoveries of Hydrochlorothiazide and Candesartan- cilexetil were found to be 101.5% and 100.9%, respectively. The proposed method was validated and successfully applied to the estimation of Hydrochlorothiazide and Candesartan cilexetil in combined tablet dosage forms.