DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF DOXOFYLINE AND SERTRALINE IN PURE BULK AND PHARMACEUTICAL DOSAGE FORMS

K. Vikram*, R.V. Valli Kumari

A simple, Accurate, precise method was developed for the simultaneous estimation of the Doxofylline and Sertaline in Tablet dosage form. Retention time of Doxofylline and Sertaline were found to be 2.3min and 3.7min. %RSD of the Doxofylline and Sertaline were and found to be 0.98 and 1.14 respectively. %assay r was Obtained as 99.71% and 99.53% for Doxofylline and Sertaline respectively. LOD, LOQ values are obtained from regression equations of Doxofylline and Sertaline were 0.09ppm, 0.26ppm and 0.44ppm, 1.34ppm respectively. Regression equation of Doxofylline is of Sertaline y = 14573x + 385.4 And y = 33848x + 4535. Retention times are decreased and that run time was decreased so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.