DEVELOPMENT OF A SIMPLE HPLC METHOD USING CORE-SHELL PARTICLES COLUMN FOR QUANTIFICATION OF IR3535® IN INSECT REPELLENT LIQUID FORMULATIONS

Sinh Thai Nguyen, Jean-Paul Salvi, Elodie Gilbert, Celine Serre, Fabrice Pirot, Roselyne Boulieu*

A simple, fast, precise and sensitive method for the quantification of IR3535^®, an insect repellent used in topical formulations, was developed by reversed-phase high performance liquid chromatography. The separation was carried out by using a core-shell silica particles column (Kinetex^® C18, 5 m, 4.6 mm x 150 mm) with a mobile phase consisting of 0.1% formic acid and methanol (40 : 60 v/v) in an isocratic mode at 0.5 ml/min and UV detection at 220 nm. The calibration curve was linear from 10 to 400 µM, with regression coefficient r² = 0.9999. Limit of detection (LOD) and Limit of quantification (LOQ) were found to be 1 and 10 M, respectively. Precision and accuracy fulfilled the acceptance criteria. This method was validated and may be routinely used for quantitative analysis of IR3535^® from various topical insect repellent liquid formulations.