EVALUATION OF INVITRO ANTICANCER AND ANTI INFLAMMATORY POTENTIAL OF MITRACARPUS VILLOSUS (Sw) DC.

K. Poonkodi*, K. Vimala devi, M. Rubini, R. Priya dharsini and S. Vinitha

The present study examines the in vitro anti inflammatory potential of ethanol and petroleum ether extracts of Mitracarpus villosus by HRBC stabilization method. In addition to that invitro anti cancer potential of ethanol extract of the M.villosus was evaluated by MTT assay. The results revealed that the petroleum ether and ethanol extracts has significant anti inflammatory activities compared to the standard. The petroleum ether extract showed the percentage of lysis of about 25.64 for 20µl followed by 36.25, 47.21 for 40 µl and 60 µl respectively. The ethanol extract showed % of lysis of 54.24, 66.17 and 78.71 for 20µl, 40 µl and 60 µl respectively. The ethanol extract showed very good percentage of membrane lysis in all the concentrations employed. The ethanol extract was tested for its anticancer potential against MCF-7-Human breast cancer cell line and HEK- 293-Human embryonic kidney cell line by MTT assay. The ethanol extract showed significant cytotoxic effect on the MCF-7 and HEK-293 cancer cell lines in dose dependant pattern and the IC50 values were determined as 217.6 and 196.8 μg/ml, respectively. This is the first report of its kind to test the ethanol extract of M.villosus for anticancer activity.