Evaluation of the Anti-cancer Activity of most potent Ethanolic fraction of Prunus avium on EAC Cells in RPMI 1640

Lopamudra Roy, Mounamukhar Bhattacharjee*

Our present study aims to determine the most potent fraction of ethanolic extract of Prunus aviun as well as to evaluate the anticancer activity of most potent fraction on EAC cells in long time incubation (by using RPMI 1640 media). Ethanolic extract of the fruit was prepared by using maceration (70% C₂H₅OH + 30% H₂0) for 10 days followed by solvent evaporation by rotary vacuum evaporator. Most potent fraction was determined by fractionation by using Chloroform, n-butanol and ethyl acetate followed by qualitative and quantitative analysis. Most potent fraction was introduced into fresh collected and sufficiently diluted EAC cells to check the cell viability under microscope by using RPMI 1640 medium. Results of fractionation shows ethyl acetate fraction is probably most potential fraction due to presence highest amount of flavonoid and phenolic compounds than the other fractions. On addition of ethyl acetate fraction to fresh collected and diluted EAC cells, it shows IC₅₀ values 43.03 ± 0.54 μg/ml, 34.62 ± 0.21 μg/ml and 26.94 ± 0.22 μg/ml respectively on 2 hr, 4hr and 8 hrs of incubation by using RPMI 1640 medium. Results indicates that the ethyl acetate fraction of ethanolic extract of Prunus avium is the most potential fraction and it has good anti-cancer activity in long time incubation as well. Still future studies can be performed to isolate the bioactive principles.