Evaluation of Total condensed tannin content and anthelmintic activities of organic extracts of four Bangladeshi plants on Tubifex tubifex worm using in vitro method

Mohammad Shah Hafez Kabir, Shabbir Ahmad, Md. Sofi Mahamoud, Md. Abdullah Al Masum, A.T.M. Mostafa Kamal, Md. Akramul Hoque, Nishan Chakrabarty, Abul Hasanat *, Md. Adnan

To explore the therapeutic effects of methanol extract of roots of Curculigo recurvata W. T. Aiton (Satipata), leaf of Amorphophallus bulbifer (Roxb.) Bl. (Olkachu), leaf of Phyllanthus sikkimensis Muell. Arg. (Dalpata) and whole plant of Thunbergia grandiflora Roxb. (Nillata) in anthelmintic (in vitro) and to determine their total condensed tannin content. Roots of C. recurvata , leaf of A. bulbifer, leaf of P. sikkimensis and whole plant of T. grandiflora was extracted with pure methanol, which are tested for anthelmintic activity on aquarium worm Tubifex tubifex by using three concentrations viz., 2.5, 5 and 10 mg/ml of each. Total condensed tannin content determined based on the procedure of Oyedemi et al (2010). Among the four extract, P. sikkimensis exhibited strong anthelmintic activity in vitro. Where it paralyzed (6.48±0.09 min) and produced death (12.26±0.65 min) of the Tubifex tubifex at 10 mg/ml dose near the value of the standard, Levamisole (3.3±0.38 min and 6.5±0.76 min) at 1 mg/ml. The content of condensed tannin well to moderate at all, but P. sikkimensis (45.14±0.5mg catechin/g) contained highest among them. For both of experiment, activity found as follows, P. sikkimensis > A. bulbifer > T. grandiflora > C. recurvata. These findings suggest that P. sikkimensis and A. bulbifer among the four plants may be potential source for the development of new anthelmintic and condensed tannin may one of such phytochemical, which exhibit anthelmintic activity.