Evalution of Anti-Inflammatory Activity of Tulsi Stem (Ocimum Sanctum Linn.) by Using Carrageenan-Induced Paw Edema Model Care

Aanchal Gwari*, Arvind Negi and Manish Mishra

Ocimum sanctum (L.) is commonly used as a herbal remedy for various ailments. But the scientific basis for its medicinal use is unknown, especially in pain and inflammation. Therefore, the present study aimed to investigate the anti-inflammatory effects of Ocimum sanctum stems in laboratory animals. Inflammation remains an area of great research interest, possibly due to the unavailability of a safer and more effective anti-inflammatory agent. This has led to an increased demand for natural products with anti-inflammatory activity, which has fewer side effects. Non-Steroidal Anti- Inflammatory Drugs (NSAIDs) such as indomethacin are used to treat inflammation, fever, and pain. However, NSAIDs cause gastric damage as a major adverse reaction. Methanol, ethanol extract from O. sanctum stem was used to study the acute effect of inflammation in rats using a carrageenan-induced paw edema model in wistar albino rats. The ethanolic extract of O. sanctum showed more impact at doses of 200 mg/kg of body weight at the fourth hour of the study shown dose-dependent and anti-inflammatory activity in acute inflammation (carrageenan-induced hind paw edema, p<0.05)) of inflammation. Hence, present investigation established some pharmacological evidences to support the folklore claim that Ocimum sanctum is used as anti-inflammatory agent.