HEPATOPROTECTIVE ACTIVITY OF ROOTS OF LAWSONIA INERMIS AGAINST PARACETAMOL AND ANTI-TUBERCULAR DRUGS INDUCED HEPATOTOXICITY IN RATS

Ravishah S1, Manjula SN, Mruthunjaya K, Krishnanand P, Pramod Chakravarthy KN, Madhu Raghav M, Javia Sweety and Mina Basirian

The present study was carried out to evaluate the hepatoprotective activity of roots of Lawsonia inermis (LI) ethanolic extract in paracetamol and anti tubercular drugs induced hepatotoxicity in Wistar rats. Roots of LI were extracted with alcohol and water which has given ethanolic extract of LI & aqueous extract of LI. Preliminary phytochemical tests were done. The in vitro hepatoprotective activity of the ethanolic extract of lawsonia inermis (LIALC) and aqueous extract of lawsonia inermis (LIAQ) were assessed. The in vivo hepatoprotective activity of LIALC was investigated against Paracetamol and Anti-TB drugs induced hepatotoxicity in Rats. Phytochemical analysis revealed presence of lactones, flavonoids, sterols, terpenes, carbohydrates, tannins compounds, which have been known for their hepatoprotective activities. In both in vivo and in vitro hepatoprotective models, the levels of cytosolic enzymes, a marker of oxidative damage to hepatocytes, were significantly reversed to almost to normal in dose dependent manner. Both the extracts significantly increased the levels of endogenous antioxidant enzymes: superoxide dismutase (SOD), catalase and glutathione (GSH) as compared to control. LI possesses marked hepatoprotective activity against paracetamol and anti tubercular drugs induced hepatotoxicity in rats as evidenced by both in vivo and in vitro results. The activity may be attributed to the individual or combined action of phytoconstituents present in it.