QUANTITATIVE ESTIMATION OF DNA ISOLATED FROM LEAVES AND STEMS OF COLEUS AROMATICUS

Soni Himesh, Singhai AK and Sharma Sarvesh

Medicinal plants play a vital role to preserve human health. The genus, Coleus consists of herbs, that are widespread in all over India and represents highly valuable plant species having therapeutic and neutraceutical importance. Genetic variation is essential for long term survival of species and it is a critical feature in conservation. For efficient conservation and management, the genetic composition of the species in different geographic locations needs to be assessed. Plants are attracting more attention among contemporary pharmacy scientists because some human diseases resulting from antibiotic resistance have gained worldwide concern. A number of methods are available and are being developed for the isolation of nucleic acids from plants. The different parts of Coleus aromaticus were studied for their nucleic acid content by using spectrophotometric analysis. In order to measure DNA content of the Leaves and stems of C.aromaticus, Spectrophotometry serves various advantages i.e. non-destructive and allows the sample to be recovered for further analysis or manipulation. Spectrophotometry uses the fact that there is a relationship between the absorption of ultraviolet light by DNA/RNA and its concentration in a sample. This article deals with modern approaches to develop a simple, efficient, reliable and cost-effective method for isolation, separation and estimation of total genomic DNA from various parts of the same species.